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北京石油化工学院2026年研究生招生接收调剂公告
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evebobo

铜虫 (小有名气)

[求助] 有关序列提交 已有1人参与

本人提交一段DNA序列,以前提交很容易,可是这次提交很奇怪各种问题,序列里面含三段cDNA,location里面不知道怎么弄了,怎么填写都不对,总是出现Stop codon '*' is found in the range.ORF查了,CDS也查了,关于DDBJ里面的location到底应该怎么填写呢???
这是搜索的信息,但不知道自己的是哪一类,是不是从ORF里面找start/stop codon,然后(。。)括出来三段呢,还是怎么弄? 有没有高手懂啊 ??
340..565        Points to a continuous range of bases bounded by and including bases 340 and 565.
complement(261..457)        Points to a continuous range of bases bounded by and including bases 261 and 457 on complementary strand.
<345..500        Indicates that the location begins at some base previous to base 345 and continues to and includes base 500. The 5'-end point of a feature is not included in the presented sequence, or the exact lower 5'-end point of a feature than base 345 is unknown.
345..>500        Indicates that the location begins and includes base 345 and continues to beyond base 500. The 3'-end point of a feature is not included in the presented sequence, or the exact higher 3'-end point of a feature than base 500 is unknown.
<345..>500        The both of 5'-end point and 3'-end point of a feature are not included in the presented sequence, or the both of exact 5'-end point and 3'-end point of a feature are unknown.
join(12..78,134..202)        Regions 12 to 78 and 134 to 202 should be joined to form one contiguous sequence
complement(join(12..78,134..202))        Regions 12 to 78 and 134 to 202 sequence on complementary strand should be joined to form one contiguous sequence.
467        Points to a single base 467 in the presented sequence.
123^124        Points to a site between bases 123 and 124

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[ Last edited by silicare on 2014-2-19 at 11:33 ]
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forrestgump

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【答案】应助回帖

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感谢参与,应助指数 +1
gyesang: 金币+2, 鼓励回帖交流经验! 2014-02-19 17:32:55
你这个我也碰到过,没有关系,你注释文献报道的那个阅码框就行了,不是所有的ATG就是起始密码子。
2楼2014-02-19 14:40:56
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evebobo

铜虫 (小有名气)

引用回帖:
2楼: Originally posted by forrestgump at 2014-02-19 14:40:56
你这个我也碰到过,没有关系,你注释文献报道的那个阅码框就行了,不是所有的ATG就是起始密码子。

可是我应该怎么提交呢? 现在问题是这种情况提交不了。。。

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3楼2014-02-20 12:47:45
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evebobo

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有人能回复下吗???谢了

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4楼2014-02-24 10:33:55
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