24СʱÈÈÃŰæ¿éÅÅÐаñ    

Znn3bq.jpeg
±±¾©Ê¯ÓÍ»¯¹¤Ñ§Ôº2026ÄêÑо¿ÉúÕÐÉú½ÓÊÕµ÷¼Á¹«¸æ
²é¿´: 2147  |  »Ø¸´: 7

lairongpeng

Òø³æ (ÕýʽдÊÖ)

[ÇóÖú] Çó½Ì¾­³£¿´Ó¢ÎÄÎÄÏ׵ĴóÉñ£¬ÕâЩÊÇɶÒâË¼ÄØ£¿

pyrG89; wA3; pyroA4 [pyr4 + copA::gfp]
(pyrG89; pyroA4; nkuA::argB)
pAL12-Lifeact [Ptef-1::Lifeact-TagRFP-T::nat1]  
sepA::sGFPpyrG89;argB2;¦¤bemA::argB;sE15,fwA1(¦¤nkuB::pyrG;nkuA;argB;uaY9)
»Ø¸´´ËÂ¥

» ²ÂÄãϲ»¶

» ±¾Ö÷ÌâÏà¹Ø¼ÛÖµÌùÍÆ¼ö£¬¶ÔÄúͬÑùÓаïÖú:

ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

jlc0225

ÖÁ×ðľ³æ (ÖøÃûдÊÖ)

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

¸Ðл²ÎÓ룬ӦÖúÖ¸Êý +1
http://www.nature.com/nprot/jour ... ot.2007.112_T2.html
Pyridoxine (ANM plus NH4, riboflavin and biotin) medium allows growth (pyroA+) versus no growth (pyroA4) to be scored
¿ªÊ¼ÐµÄѧϰ£¡
2Â¥2013-09-03 15:48:03
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

lairongpeng

Òø³æ (ÕýʽдÊÖ)

ÒýÓûØÌû:
2Â¥: Originally posted by jlc0225 at 2013-09-03 15:48:03
http://www.nature.com/nprot/journal/v2/n4/fig_tab/nprot.2007.112_T2.html
Pyridoxine (ANM plus NH4, riboflavin and biotin) medium allows growth (pyroA+) versus no growth (pyroA4) to be scored...

Äܲ»ÄÜÂé·³Äã½âÊÍÒ»ÏÂÄØ£¿±ÈÈçÕâÊÇÔÚһƪPNASÎÄÏ×Öп´µ½µÄ¡£²»ÖªµÀʲôÒâ˼£ºThe A. nidulans reference strain was FGSC 4A. Gene deletions in A. nidulans were carried out by transforming FGSC A1149 (pyrG89; pyroA4; nkuA::argB) by established protocols.ÆäÖУ¬(pyrG89; pyroA4; nkuA::argB) ÊÇʲôÒâË¼ÄØ£¿
3Â¥2013-09-03 19:30:28
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

jlc0225

ÖÁ×ðľ³æ (ÖøÃûдÊÖ)

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

¡ï ¡ï ¡ï
kx444555: ½ð±Ò+3, ¹ÄÀø½»Á÷ 2013-09-04 15:23:07
http://www.fgsc.net/scripts/Stra ... age.asp?OrgID=11562
Lesion:         pyrG89        pyrimidine req. orotidine MP decarboxylase
Enzyme Name: orotidine 5'-monophosphate decarboxylase
Lesion Information for Marker:
      Linkage Group of pyrG89: IL
Marker description or requirements:
      Optimal supplementation is 2.5 mg/ml (10 mM) each of uridine + uracil. pyrG89is cold sensitive and sectors
Reference for: pyrG89: 10167. de Souza et al. 1998. Curr. Genet. 33,60-69
Reference for: pyrG89: 10472. Oakley, B. R. et al. 1987. Gene 61;385-399
Reference for: pyrG89: 10486. Palmer, L. M. & D. J. Cove 1975. Mol. Gen. Genet. 138,243-255.
Lesion:         pyroA4        pyridoxine req.
Lesion Information for Marker:
      Linkage Group of pyroA4: IVR
Marker description or requirements:
      Responds to pyridoxine
Reference for: pyroA4: 10332. Kafer, E. 1958. Adv. Genet. , 9,105.
Lesion:         nkuA::argB       
Marker description or requirements:
      Arginine requirement. The nkuA gene was deleted in KJ12 by replacing it with argB. This was crossed to A770. TN02A7 is a segregant of this cross. Deletion of the nkuA (the A. nidulans homolog of human Ku70 gene) reduces the frequency of non-homologous integration of transforming DNA and greatly facilitates gene targeting. NkuA deleted strains show no obvious growth defect and are not sensitive to a variety of DNA damaging drugs.
¿ªÊ¼ÐµÄѧϰ£¡
4Â¥2013-09-04 09:07:52
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

jlc0225

ÖÁ×ðľ³æ (ÖøÃûдÊÖ)

ÔڹȸèÀïËÑ¡°FGSC A1149¡±µÃµ½µÄ´ð°¸
¿ªÊ¼ÐµÄѧϰ£¡
5Â¥2013-09-04 09:08:59
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

ÄäÃû

Óû§×¢Ïú (ÕýʽдÊÖ)

¡ï ¡ï ¡ï
¸Ðл²ÎÓ룬ӦÖúÖ¸Êý +1
kx444555: ½ð±Ò+3, ¹ÄÀø½»Á÷ 2013-09-04 15:23:14
±¾Ìû½öÂ¥Ö÷¿É¼û
6Â¥2013-09-04 13:55:57
ÒÑÔÄ   ÉêÇëMolEPI   »Ø¸´´ËÂ¥   ±à¼­   ²é¿´ÎÒµÄÖ÷Ò³

lairongpeng

Òø³æ (ÕýʽдÊÖ)

ллÄúµÄ°ïÖú£¬Ð»Ð»£¡
7Â¥2013-09-11 18:56:04
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

ËÎΰͬѧ

Í­³æ (ÖøÃûдÊÖ)

8Â¥2015-03-01 20:18:48
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
Ïà¹Ø°æ¿éÌø×ª ÎÒÒª¶©ÔÄÂ¥Ö÷ lairongpeng µÄÖ÷Ìâ¸üÐÂ
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼ÑÐ] »¯Ñ§¹¤³Ìµ÷¼Á289 +22 yangæÃ 2026-04-07 23/1150 2026-04-07 22:49 by À´¿´Á÷ÐÇÓê10
[¿¼ÑÐ] 278Çóµ÷¼Á +13 ·¶æÃÄÈ 2026-04-07 14/700 2026-04-07 22:42 by À´¿´Á÷ÐÇÓê10
[¿¼ÑÐ] ²ÄÁÏר˶(0856) 339·ÖÇóµ÷¼Á +14 ¹þ¹þ¹þ¶ì¹þ¹þ¹þ 2026-04-05 14/700 2026-04-07 22:02 by lijunpoly
[¿¼ÑÐ] ÉúÎïµ÷¼Á +5 ³È×Ó³È×Ó³È×Ó°¡ 2026-04-05 9/450 2026-04-07 15:31 by Éϰ¶¿ì¿ì
[¿¼ÑÐ] 334·Ö»úеר˶Çóµ÷¼Á +3 µ°»¨×ϲËÌÀ 2026-04-03 3/150 2026-04-07 14:49 by åÐÒ£cocoa
[¿¼ÑÐ] 22408 331·ÖÇóµ÷¼Á +4 y__1 2026-04-06 4/200 2026-04-06 17:26 by ÍÁľ˶ʿÕÐÉú
[¿¼ÑÐ] 0854Çóµ÷¼Á +4 assdll 2026-04-05 4/200 2026-04-06 12:29 by ÖзÉÔº¿Õ¹ÜѧԺÑ
[¿¼ÑÐ] Çóµ÷¼Á +10 chenxrlkx 2026-04-05 10/500 2026-04-06 11:31 by Öí»á·É
[¿¼ÑÐ] Ò»Ö¾Ô¸Î人Àí¹¤´óѧ-085601²ÄÁϹ¤³Ì£¨×¨Ë¶£©-×Ü·Ö353Çóµ÷¼Á +3 2626262626li 2026-04-02 3/150 2026-04-06 09:08 by Î޼ʵIJÝÔ­
[¿¼ÑÐ] 296Çóµ÷¼Á +3 Íô£¡£¿£¡ 2026-04-05 5/250 2026-04-05 17:38 by À¶ÔÆË¼Óê
[¿¼ÑÐ] ÄÐÉú£¬Ò»Ö¾Ô¸»¦9ÉúÎïѧ071000£¬³õÊÔ308Çóµ÷¼Á +3 Áõīī 2026-04-04 3/150 2026-04-05 08:26 by barlinike
[¿¼ÑÐ] 294Çóµ÷¼Á +6 Grey_Ey 2026-04-02 9/450 2026-04-04 22:07 by hemengdong
[¿¼ÑÐ] 268Çóµ÷¼Á +8 ÄãºÃtg 2026-04-03 9/450 2026-04-04 05:08 by gswylq
[¿¼ÑÐ] 372·Ö²ÄÁÏÓ뻯¹¤£¨085600£©Ò»Ö¾Ô¸ºþÄÏ´óѧÇóµ÷¼Á +3 À¶¼ãƬ 2026-04-03 4/200 2026-04-03 17:58 by Jimmyandyou
[¿¼ÑÐ] 081200-11408-276ѧ˶Çóµ÷¼Á +5 ´Þwj 2026-04-03 5/250 2026-04-03 15:06 by arrow8852
[¿¼ÑÐ] ר˶085601Çóµ÷¼Á +7 suyifei 2026-04-03 8/400 2026-04-03 14:00 by ÐÀϲ777
[¿¼ÑÐ] Ò»Ö¾Ô¸±±¾©½»Í¨´óѧ²ÄÁϹ¤³Ì×Ü·Ö358 +4 cs0106 2026-04-03 4/200 2026-04-03 13:41 by °ÙÁéͯ888
[¿¼ÑÐ] µ÷¼Á +7 ìíáº. 2026-04-02 7/350 2026-04-03 09:11 by »¨ß»¹Ç·600
[¿¼ÑÐ] 372·Ö²ÄÁÏÓ뻯¹¤£¨085600£©Ò»Ö¾Ô¸ºþÄÏ´óѧÇóµ÷¼Á +5 À¶¼ãƬ 2026-04-02 6/300 2026-04-02 21:37 by dongzh2009
[¿¼ÑÐ] ÍÁľ304Çóµ÷¼Á +4 ÍÃͻͻͻ£¬ 2026-04-02 5/250 2026-04-02 21:16 by ÍÃͻͻͻ£¬
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û