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lairongpeng

银虫 (正式写手)

[求助] 求教经常看英文文献的大神,这些是啥意思呢?

pyrG89; wA3; pyroA4 [pyr4 + copA::gfp]
(pyrG89; pyroA4; nkuA::argB)
pAL12-Lifeact [Ptef-1::Lifeact-TagRFP-T::nat1]  
sepA::sGFPpyrG89;argB2;ΔbemA::argB;sE15,fwA1(ΔnkuB::pyrG;nkuA;argB;uaY9)
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jlc0225

至尊木虫 (著名写手)

在谷歌里搜“FGSC A1149”得到的答案
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5楼2013-09-04 09:08:59
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jlc0225

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【答案】应助回帖

感谢参与,应助指数 +1
http://www.nature.com/nprot/jour ... ot.2007.112_T2.html
Pyridoxine (ANM plus NH4, riboflavin and biotin) medium allows growth (pyroA+) versus no growth (pyroA4) to be scored
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2楼2013-09-03 15:48:03
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lairongpeng

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引用回帖:
2楼: Originally posted by jlc0225 at 2013-09-03 15:48:03
http://www.nature.com/nprot/journal/v2/n4/fig_tab/nprot.2007.112_T2.html
Pyridoxine (ANM plus NH4, riboflavin and biotin) medium allows growth (pyroA+) versus no growth (pyroA4) to be scored...

能不能麻烦你解释一下呢?比如这是在一篇PNAS文献中看到的。不知道什么意思:The A. nidulans reference strain was FGSC 4A. Gene deletions in A. nidulans were carried out by transforming FGSC A1149 (pyrG89; pyroA4; nkuA::argB) by established protocols.其中,(pyrG89; pyroA4; nkuA::argB) 是什么意思呢?
3楼2013-09-03 19:30:28
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jlc0225

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【答案】应助回帖

★ ★ ★
kx444555: 金币+3, 鼓励交流 2013-09-04 15:23:07
http://www.fgsc.net/scripts/Stra ... age.asp?OrgID=11562
Lesion:         pyrG89        pyrimidine req. orotidine MP decarboxylase
Enzyme Name: orotidine 5'-monophosphate decarboxylase
Lesion Information for Marker:
      Linkage Group of pyrG89: IL
Marker description or requirements:
      Optimal supplementation is 2.5 mg/ml (10 mM) each of uridine + uracil. pyrG89is cold sensitive and sectors
Reference for: pyrG89: 10167. de Souza et al. 1998. Curr. Genet. 33,60-69
Reference for: pyrG89: 10472. Oakley, B. R. et al. 1987. Gene 61;385-399
Reference for: pyrG89: 10486. Palmer, L. M. & D. J. Cove 1975. Mol. Gen. Genet. 138,243-255.
Lesion:         pyroA4        pyridoxine req.
Lesion Information for Marker:
      Linkage Group of pyroA4: IVR
Marker description or requirements:
      Responds to pyridoxine
Reference for: pyroA4: 10332. Kafer, E. 1958. Adv. Genet. , 9,105.
Lesion:         nkuA::argB       
Marker description or requirements:
      Arginine requirement. The nkuA gene was deleted in KJ12 by replacing it with argB. This was crossed to A770. TN02A7 is a segregant of this cross. Deletion of the nkuA (the A. nidulans homolog of human Ku70 gene) reduces the frequency of non-homologous integration of transforming DNA and greatly facilitates gene targeting. NkuA deleted strains show no obvious growth defect and are not sensitive to a variety of DNA damaging drugs.
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4楼2013-09-04 09:07:52
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