24小时热门版块排行榜    

查看: 2106  |  回复: 7
当前只显示满足指定条件的回帖,点击这里查看本话题的所有回帖

lairongpeng

银虫 (正式写手)

[求助] 求教经常看英文文献的大神,这些是啥意思呢?

pyrG89; wA3; pyroA4 [pyr4 + copA::gfp]
(pyrG89; pyroA4; nkuA::argB)
pAL12-Lifeact [Ptef-1::Lifeact-TagRFP-T::nat1]  
sepA::sGFPpyrG89;argB2;ΔbemA::argB;sE15,fwA1(ΔnkuB::pyrG;nkuA;argB;uaY9)
回复此楼

» 猜你喜欢

» 本主题相关价值贴推荐,对您同样有帮助:

已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

jlc0225

至尊木虫 (著名写手)

【答案】应助回帖

★ ★ ★
kx444555: 金币+3, 鼓励交流 2013-09-04 15:23:07
http://www.fgsc.net/scripts/Stra ... age.asp?OrgID=11562
Lesion:         pyrG89        pyrimidine req. orotidine MP decarboxylase
Enzyme Name: orotidine 5'-monophosphate decarboxylase
Lesion Information for Marker:
      Linkage Group of pyrG89: IL
Marker description or requirements:
      Optimal supplementation is 2.5 mg/ml (10 mM) each of uridine + uracil. pyrG89is cold sensitive and sectors
Reference for: pyrG89: 10167. de Souza et al. 1998. Curr. Genet. 33,60-69
Reference for: pyrG89: 10472. Oakley, B. R. et al. 1987. Gene 61;385-399
Reference for: pyrG89: 10486. Palmer, L. M. & D. J. Cove 1975. Mol. Gen. Genet. 138,243-255.
Lesion:         pyroA4        pyridoxine req.
Lesion Information for Marker:
      Linkage Group of pyroA4: IVR
Marker description or requirements:
      Responds to pyridoxine
Reference for: pyroA4: 10332. Kafer, E. 1958. Adv. Genet. , 9,105.
Lesion:         nkuA::argB       
Marker description or requirements:
      Arginine requirement. The nkuA gene was deleted in KJ12 by replacing it with argB. This was crossed to A770. TN02A7 is a segregant of this cross. Deletion of the nkuA (the A. nidulans homolog of human Ku70 gene) reduces the frequency of non-homologous integration of transforming DNA and greatly facilitates gene targeting. NkuA deleted strains show no obvious growth defect and are not sensitive to a variety of DNA damaging drugs.
开始新的学习!
4楼2013-09-04 09:07:52
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
查看全部 8 个回答

jlc0225

至尊木虫 (著名写手)

【答案】应助回帖

感谢参与,应助指数 +1
http://www.nature.com/nprot/jour ... ot.2007.112_T2.html
Pyridoxine (ANM plus NH4, riboflavin and biotin) medium allows growth (pyroA+) versus no growth (pyroA4) to be scored
开始新的学习!
2楼2013-09-03 15:48:03
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

lairongpeng

银虫 (正式写手)

引用回帖:
2楼: Originally posted by jlc0225 at 2013-09-03 15:48:03
http://www.nature.com/nprot/journal/v2/n4/fig_tab/nprot.2007.112_T2.html
Pyridoxine (ANM plus NH4, riboflavin and biotin) medium allows growth (pyroA+) versus no growth (pyroA4) to be scored...

能不能麻烦你解释一下呢?比如这是在一篇PNAS文献中看到的。不知道什么意思:The A. nidulans reference strain was FGSC 4A. Gene deletions in A. nidulans were carried out by transforming FGSC A1149 (pyrG89; pyroA4; nkuA::argB) by established protocols.其中,(pyrG89; pyroA4; nkuA::argB) 是什么意思呢?
3楼2013-09-03 19:30:28
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

jlc0225

至尊木虫 (著名写手)

在谷歌里搜“FGSC A1149”得到的答案
开始新的学习!
5楼2013-09-04 09:08:59
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
最具人气热帖推荐 [查看全部] 作者 回/看 最后发表
[考研] 271材料工程求调剂 +4 .6lL 2026-03-18 4/200 2026-03-18 20:05 by 楤哥
[考研] 0703化学调剂,求各位老师收留 +9 秋有木北 2026-03-14 9/450 2026-03-18 19:49 by macy2011
[考研] 085601材料工程专硕求调剂 +6 慕寒mio 2026-03-16 6/300 2026-03-18 14:26 by 007_lilei
[考研] 能源材料化学课题组招收硕士研究生8-10名 +4 脱颖而出 2026-03-16 9/450 2026-03-18 14:13 by 脱颖而出
[考研] 工科材料085601 279求调剂 +6 困于星晨 2026-03-17 6/300 2026-03-18 10:21 by kkcoco25
[考研] 293求调剂 +11 zjl的号 2026-03-16 16/800 2026-03-18 08:10 by zhukairuo
[考博] 26博士申请 +3 1042136743 2026-03-17 3/150 2026-03-17 23:30 by 轻松不少随
[考研] 302求调剂 +9 负心者当诛 2026-03-11 9/450 2026-03-17 17:13 by ruiyingmiao
[考研] 本人考085602 化学工程 专硕 +16 不知道叫什么! 2026-03-15 18/900 2026-03-17 17:05 by ruiyingmiao
[考研] 285化工学硕求调剂(081700) +9 柴郡猫_ 2026-03-12 9/450 2026-03-17 10:18 by Sammy2
[考研] 278求调剂 +3 Yy7400 2026-03-13 3/150 2026-03-17 08:24 by laoshidan
[考研] 070300化学学硕求调剂 +6 太想进步了0608 2026-03-16 6/300 2026-03-16 16:13 by kykm678
[考研] 288求调剂 +4 奇点0314 2026-03-14 4/200 2026-03-14 23:04 by JourneyLucky
[考研] 材料与化工 323 英一+数二+物化,一志愿:哈工大 本人本科双一流 +4 自由的_飞翔 2026-03-13 5/250 2026-03-14 19:39 by hmn_wj
[考研] 297一志愿上交085600求调剂 +5 指尖八千里 2026-03-14 5/250 2026-03-14 17:26 by a不易
[考研] 308 085701 四六级已过求调剂 +7 温乔乔乔乔 2026-03-12 14/700 2026-03-14 10:49 by JourneyLucky
[考研] 材料与化工(0856)304求B区调剂 +6 邱gl 2026-03-12 7/350 2026-03-13 23:24 by 邱gl
[考研] [0860]321分求调剂,ab区皆可 +4 宝贵热 2026-03-13 4/200 2026-03-13 22:01 by 星空星月
[考研] 材料301分求调剂 +5 Liyouyumairs 2026-03-12 5/250 2026-03-13 14:42 by JourneyLucky
[考研] 333求调剂 +3 152697 2026-03-12 4/200 2026-03-13 07:08 by Iveryant
信息提示
请填处理意见