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lairongpeng

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pyrG89; wA3; pyroA4 [pyr4 + copA::gfp]
(pyrG89; pyroA4; nkuA::argB)
pAL12-Lifeact [Ptef-1::Lifeact-TagRFP-T::nat1]  
sepA::sGFPpyrG89;argB2;¦¤bemA::argB;sE15,fwA1(¦¤nkuB::pyrG;nkuA;argB;uaY9)
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lairongpeng

Òø³æ (ÕýʽдÊÖ)

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7Â¥2013-09-11 18:56:04
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jlc0225

ÖÁ×ðľ³æ (ÖøÃûдÊÖ)

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http://www.nature.com/nprot/jour ... ot.2007.112_T2.html
Pyridoxine (ANM plus NH4, riboflavin and biotin) medium allows growth (pyroA+) versus no growth (pyroA4) to be scored
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2Â¥2013-09-03 15:48:03
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lairongpeng

Òø³æ (ÕýʽдÊÖ)

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2Â¥: Originally posted by jlc0225 at 2013-09-03 15:48:03
http://www.nature.com/nprot/journal/v2/n4/fig_tab/nprot.2007.112_T2.html
Pyridoxine (ANM plus NH4, riboflavin and biotin) medium allows growth (pyroA+) versus no growth (pyroA4) to be scored...

Äܲ»ÄÜÂé·³Äã½âÊÍÒ»ÏÂÄØ£¿±ÈÈçÕâÊÇÔÚһƪPNASÎÄÏ×Öп´µ½µÄ¡£²»ÖªµÀʲôÒâ˼£ºThe A. nidulans reference strain was FGSC 4A. Gene deletions in A. nidulans were carried out by transforming FGSC A1149 (pyrG89; pyroA4; nkuA::argB) by established protocols.ÆäÖУ¬(pyrG89; pyroA4; nkuA::argB) ÊÇʲôÒâË¼ÄØ£¿
3Â¥2013-09-03 19:30:28
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jlc0225

ÖÁ×ðľ³æ (ÖøÃûдÊÖ)

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kx444555: ½ð±Ò+3, ¹ÄÀø½»Á÷ 2013-09-04 15:23:07
http://www.fgsc.net/scripts/Stra ... age.asp?OrgID=11562
Lesion:         pyrG89        pyrimidine req. orotidine MP decarboxylase
Enzyme Name: orotidine 5'-monophosphate decarboxylase
Lesion Information for Marker:
      Linkage Group of pyrG89: IL
Marker description or requirements:
      Optimal supplementation is 2.5 mg/ml (10 mM) each of uridine + uracil. pyrG89is cold sensitive and sectors
Reference for: pyrG89: 10167. de Souza et al. 1998. Curr. Genet. 33,60-69
Reference for: pyrG89: 10472. Oakley, B. R. et al. 1987. Gene 61;385-399
Reference for: pyrG89: 10486. Palmer, L. M. & D. J. Cove 1975. Mol. Gen. Genet. 138,243-255.
Lesion:         pyroA4        pyridoxine req.
Lesion Information for Marker:
      Linkage Group of pyroA4: IVR
Marker description or requirements:
      Responds to pyridoxine
Reference for: pyroA4: 10332. Kafer, E. 1958. Adv. Genet. , 9,105.
Lesion:         nkuA::argB       
Marker description or requirements:
      Arginine requirement. The nkuA gene was deleted in KJ12 by replacing it with argB. This was crossed to A770. TN02A7 is a segregant of this cross. Deletion of the nkuA (the A. nidulans homolog of human Ku70 gene) reduces the frequency of non-homologous integration of transforming DNA and greatly facilitates gene targeting. NkuA deleted strains show no obvious growth defect and are not sensitive to a variety of DNA damaging drugs.
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4Â¥2013-09-04 09:07:52
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