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lijh1234

木虫 (小有名气)

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虫号: 770633
注册: 2009-05-15
性别: GG
专业: 慢性阻塞性肺疾病

[求助] 过表达基因序列---腺病毒包装用

我要做一个基因的过表达腺病毒包装，该基因表达的蛋白为（precursor），在细胞内会被切成N端和C端。但我只想要N端的序列，请问这个怎么查。
我在NCBI上查到了其蛋白的信息，但有些地方不大懂

FEATURES          Location/Qualifiers
   source       1..437
                  /organism="Mus musculus"
                  /strain="C57BL/6"
                  /db_xref="taxon:10090"
                  /chromosome="5"
                  /map="5 14.39 cM"
   Protein       1..437
                  /product="sonic hedgehog protein precursor"
                  /note="sonic hedgehog protein; HHG-1; hemimelic extra
                  toes; short digits"
   sig_peptide    1..24
                  /inference="non-experimental evidence, no additional
                  details recorded"
                  /note="Potential; propagated from UniProtKB/Swiss-Prot
                  (Q62226.2)"
                  /calculated_mol_wt=2530
   mat_peptide    25..437
                  /product="Sonic hedgehog protein"
                  /experiment="experimental evidence, no additional details
                  recorded"
                  /note="propagated from UniProtKB/Swiss-Prot (Q62226.2)"
                  /calculated_mol_wt=45261
   Region       26..185
                  /region_name="HH_signal"
                  /note="Hedgehog amino-terminal signalling domain;
                  pfam01085"
                  /db_xref="CDD:201590"
   Region       188..423
                  /region_name="Hint"
                  /note="Hint module; pfam01079"
                  /db_xref="CDD:144609"
   Site          198..199
                  /site_type="cleavage"
                  /inference="non-experimental evidence, no additional
                  details recorded"
                  /note="Cleavage, by autolysis (By similarity); propagated
                  from UniProtKB/Swiss-Prot (Q62226.2)"
   Region       199..349
                  /region_name="Hint"
                  /note="Hedgehog/Intein domain, found in Hedgehog proteins
                  as well as proteins which contain inteins and undergo
                  protein splicing (e.g. DnaB, RIR1-2, GyrA and Pol). In
                  protein splicing an intervening polypeptide sequence - the
                  intein - is excised from a protein; cd00081"
                  /db_xref="CDD:73181"
   Site          order(199,271,348..349)
                  /site_type="other"
                  /note="protein-splicing catalytic site"
                  /db_xref="CDD:73181"
   Site          order(199,268,271)
                  /site_type="other"
                  /note="thioester formation/cholesterol transfer"
                  /db_xref="CDD:73181"
   Site          244
                  /site_type="other"
                  /inference="non-experimental evidence, no additional
                  details recorded"
                  /note="Involved in cholesterol transfer (By similarity);
                  propagated from UniProtKB/Swiss-Prot (Q62226.2)"
   Site          268
                  /site_type="other"
                  /inference="non-experimental evidence, no additional
                  details recorded"
                  /note="Involved in auto-cleavage (By similarity);
                  propagated from UniProtKB/Swiss-Prot (Q62226.2)"
   Site          271
                  /site_type="other"
                  /inference="non-experimental evidence, no additional
                  details recorded"
                  /note="Essential for auto-cleavage (By similarity);
                  propagated from UniProtKB/Swiss-Prot (Q62226.2)"
   CDS          1..437
                  /gene="Shh"
                  /gene_synonym="9530036O11Rik; Dsh; Hhg1; Hx; Hxl3;
                  M100081"
                  /coded_by="NM_009170.3:339..1652"
                  /db_xref="CCDS:CCDS19146.1"
                  /db_xref="GeneID:20423"
                  /db_xref="MGI:98297"
ORIGIN
      1 mllllarcfl vilassllvc pglacgpgrg fgkrrhpkkl tplaykqfip nvaektlgas
   61 gryegkitrn serfkeltpn ynpdiifkde entgadrlmt qrckdklnal aisvmnqwpg
   121 vklrvtegwd edghhseesl hyegravdit tsdrdrskyg mlarlaveag fdwvyyeska
   181 hihcsvkaen svaaksggcf pgsatvhleq ggtklvkdlr pgdrvlaadd qgrllysdfl
   241 tfldrdegak kvfyvietle prerllltaa hllfvaphnd sgptpgpsal fasrvrpgqr
   301 vyvvaerggd rrllpaavhs vtlreeeaga yapltahgti linrvlascy avieehswah
   361 rafapfrlah allaalapar tdgggggsip aaqsatearg aeptagihwy sqllyhigtw
   421 lldsetmhpl gmavkss
//

我在想是不是我标红的那段，就是我想要的N-terminal部分？
是不是把这段直接给公司他们就可以做出来了？

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1楼 2013-05-03 19:46:46

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