| ²é¿´: 2349 | »Ø¸´: 5 | |||
| µ±Ç°Ö»ÏÔʾÂú×ãÖ¸¶¨Ìõ¼þµÄ»ØÌû£¬µã»÷ÕâÀï²é¿´±¾»°ÌâµÄËùÓлØÌû | |||
ʯ³ÇÖ®×Óľ³æ (ÕýʽдÊÖ)
|
[ÇóÖú]
PCR-DGGEµÄÒýÎïÉè¼Æ
|
||
| ÇóÖú»·¾³ÖзÖÎöÕæ¾ú¡¢Ï¸¾ú¡¢·ÅÏß¾ú¶àÑùÐÔºÍÖÖȺ½á¹¹Ê±£¬²ÉÓÃPCR-DGGE·ÖÎöʱµÄÒýÎ×îºÃÊdz²Ê½PCRµÄÒýÎï¶Ô¡£°Ýл£¡ |
» ²ÂÄãϲ»¶
²ÄÁÏÓ뻯¹¤304ÇóBÇøµ÷¼Á
ÒѾÓÐ7È˻ظ´
0703»¯Ñ§336·ÖÇóµ÷¼Á
ÒѾÓÐ4È˻ظ´
[¸´ÊÔµ÷¼Á]Î÷ÄϿƼ¼´óѧ¹ú·À/²ÄÁϵ¼Ê¦ÍƼö
ÒѾÓÐ6È˻ظ´
»¯Ñ§¹¤³Ì321·ÖÇóµ÷¼Á
ÒѾÓÐ12È˻ظ´
211±¾£¬11408Ò»Ö¾Ô¸ÖпÆÔº277·Ö£¬ÔøÔÚÖпÆÔº×Ô¶¯»¯Ëùʵϰ
ÒѾÓÐ4È˻ظ´
²ÄÁÏר˶326Çóµ÷¼Á
ÒѾÓÐ5È˻ظ´
¶«ÄÏ´óѧ364Çóµ÷¼Á
ÒѾÓÐ5È˻ظ´
¹ú×Ô¿ÆÃæÉÏ»ù½ð×ÖÌå
ÒѾÓÐ7È˻ظ´
ҩѧ383 Çóµ÷¼Á
ÒѾÓÐ4È˻ظ´
286Çóµ÷¼Á
ÒѾÓÐ5È˻ظ´
» ±¾Ö÷ÌâÏà¹Ø¼ÛÖµÌùÍÆ¼ö£¬¶ÔÄúͬÑùÓаïÖú:
¹ØÓÚPCR-DGGE¼¼Êõ£¬´Ë¼¼ÊõÄÜ·ÖÎöÍÁÈÀÖеÄÕæ¾úÂð£¿ÓÃʲôÒýÎïºÃ£¿ÐèҪעÒâʲô£¿
ÒѾÓÐ19È˻ظ´
ÇëÎÊ´ó¼Ò×öPCR-DGGEµÄʱºò Õæ¾úϸ¾ú·ÅÏß¾ú ·Ö±ðÓÃʲôÒýÎ O(¡É_¡É)Oлл
ÒѾÓÐ13È˻ظ´
·´×ªÂ¼ÒýÎïµÄÑ¡ÔñÓëReal-Time PCRÒýÎïÉè¼ÆµÄÒªÇó
ÒѾÓÐ298È˻ظ´
ϸ¾úÒýÎï 338F 518R ×öDGGEÖ®ºó pcr
ÒѾÓÐ9È˻ظ´
¼ò²¢ÒýÎï PCR²úÎïµÄDGGE¼ì²â~~~~
ÒѾÓÐ5È˻ظ´
DGGEÒýÎreal-time PCRÒýÎïºÍ clone libraryÒýÎï µÄת»¯ÎÊÌâ
ÒѾÓÐ8È˻ظ´
¹ØÓÚ341FºÍ534RÒýÎïµÄ
ÒѾÓÐ7È˻ظ´
PCR×î¶Ì¿ÉÒÔP¶àÉÙbp£¬Çó´ó¼Ò°ïÎÒÉè¼ÆÒýÎ
ÒѾÓÐ7È˻ظ´
×öRT-PCRʱ¸ÃÓÃÄĶΰ±»ùËáÐòÁÐÉè¼ÆÒýÎï
ÒѾÓÐ6È˻ظ´
¡¾ÇóÖú/½»Á÷¡¿Ó«¹â¶¨Á¿PCRÓë°ë¶¨Á¿µÄRT£PCRÔÚÉè¼ÆÒýÎïʱÓÐÊ²Ã´Çø±ð£¿
ÒѾÓÐ8È˻ظ´
¡¾ÇóÖú/½»Á÷¡¿pCRÒýÎïÉè¼ÆµÄÏêϸ¹æÔò
ÒѾÓÐ7È˻ظ´
¡¾ÇóÖú/½»Á÷¡¿Ï¸¾úV3ÇøPCR³öÏÖÎÊÌ⣬ÒýÎïΪ357ºÍ518£¬ÓÃÓÚDGGE£¡
ÒѾÓÐ8È˻ظ´

ç÷Ñþ¶ù
½ð³æ (СÓÐÃûÆø)
- Ó¦Öú: 1 (Ó×¶ùÔ°)
- ½ð±Ò: 2362.8
- É¢½ð: 7
- Ìû×Ó: 170
- ÔÚÏß: 31.8Сʱ
- ³æºÅ: 1049495
- ×¢²á: 2010-06-29
- רҵ: »·¾³Î¢ÉúÎïѧ
¡¾´ð°¸¡¿Ó¦Öú»ØÌû
¡ï ¡ï ¡ï
¸Ðл²ÎÓ룬ӦÖúÖ¸Êý +1
zhang8826857(½ð±Ò+3): ¹ÄÀøÐ³æ 2011-12-12 15:15:24
ʯ³ÇÖ®×Ó(½ð±Ò+5): ллÁ˰¡£¬ÄÜ·ñ¸øÎÒÔ´ÎÄÏס£°Ýл°¡£¡ 2011-12-12 23:20:04
¸Ðл²ÎÓ룬ӦÖúÖ¸Êý +1
zhang8826857(½ð±Ò+3): ¹ÄÀøÐ³æ 2011-12-12 15:15:24
ʯ³ÇÖ®×Ó(½ð±Ò+5): ллÁ˰¡£¬ÄÜ·ñ¸øÎÒÔ´ÎÄÏס£°Ýл°¡£¡ 2011-12-12 23:20:04
|
Outer PCR ÒýÎCA/CT ¼ò²¢Î»µã ÕýÏòÒýÎï8fΪ(5¡ä-ggA gAg TTT gAT CA/CT ggC T-3¡ä) 20bp ·´ÏòÒýÎï798rΪ( 5¡ä-CCA ggg TAT CTA ATC CTg TT -3¡ä) 20bp Inner PCR ÒýÎ 341F-GC (5¡ä-cgc ccg ccg cgc gcg gcg ggc ggg gcg ggg gca cgg ggg gCC TAC gCg Agg CAg CAg-3¡ä) 57bp 534R (5¡ä-ATT ACC gCg gCT gCT gg-3¡ä) 17bp ÎÒÓùýÕâ¸ö |

3Â¥2011-12-12 14:56:40
xiadongliang
½ð³æ (ÕýʽдÊÖ)
- MicEPI: 3
- Ó¦Öú: 64 (³õÖÐÉú)
- ½ð±Ò: 973.3
- É¢½ð: 1299
- ºì»¨: 15
- Ìû×Ó: 759
- ÔÚÏß: 361.7Сʱ
- ³æºÅ: 433819
- ×¢²á: 2007-08-18
- ÐÔ±ð: GG
- רҵ: »·¾³Î¢ÉúÎïѧ
¡¾´ð°¸¡¿Ó¦Öú»ØÌû
¡ï
¸Ðл²ÎÓ룬ӦÖúÖ¸Êý +1
zhang8826857(½ð±Ò+1): ¹ÄÀøÓ¦Öú 2011-12-12 15:15:17
ʯ³ÇÖ®×Ó(½ð±Ò+15): ÄÜ·ñ¸ø³öÏà¹ØÎÄÏ×£¬°Ýл£¡ 2011-12-12 23:19:00
¸Ðл²ÎÓ룬ӦÖúÖ¸Êý +1
zhang8826857(½ð±Ò+1): ¹ÄÀøÓ¦Öú 2011-12-12 15:15:17
ʯ³ÇÖ®×Ó(½ð±Ò+15): ÄÜ·ñ¸ø³öÏà¹ØÎÄÏ×£¬°Ýл£¡ 2011-12-12 23:19:00
|
Õâ¶¼ÊÇͨÓÃÒýÎï¡££¨Ãâ·ÑÔùËÍ£¬¹þ¹þ£© ϸ¾ú16S rDNA V3 region 194 bp 338F£º5-CCT ACG GGA GGC AGC AG-3 518 R£º5-ATT ACC GCG GCT GCT GG-3 GC338F£º5-CGCCCGGGGCGCGCCCCGGGGCGGGGCGGGGGCGCGGGGGG CCT ACG GGA GGC AGC AG-3 8%Äý½º35%-55% 200V µçÓ¾3 h Õæ¾ú18S rDNA 340 bp FR1£º5-AIC CAT TCA ATC GGT AIT-3 FF390£º5-CGA TAA CGA ACG AGA CCT-3 FR1£º5-CGCCCGGGGCGCGCCCCGGGGCGGGGCGGGGGCGCGGGGGG AIC CAT TCA ATC GGT AIT-3 200V µçÓ¾4 h 6%Äý½º35%-55% ·ÅÏß¾ú 270 bp F243£º5- GGA TGA GCC CGC GGC CTA-3 R513£º5- CGG CCG CGG CTG CTG GCA CGT A-3 R513£º5-CGCCCGGGGCGCGCCCCGGGGCGGGGCGGGGGCGCGGGGGG CGG CCG CGG CTG CTG GCA CGT A-3 6%Äý½º35%-55% 200V µçÓ¾3.5 h |
2Â¥2011-12-12 14:29:04
mima146
Òø³æ (СÓÐÃûÆø)
- Ó¦Öú: 2 (Ó×¶ùÔ°)
- ½ð±Ò: 229.4
- É¢½ð: 20
- ºì»¨: 1
- Ìû×Ó: 195
- ÔÚÏß: 66.4Сʱ
- ³æºÅ: 1693072
- ×¢²á: 2012-03-15
- רҵ: ·¨Ò½Îï֤ѧ¡¢·¨Ò½ÈËÀàѧ
5Â¥2012-07-22 18:57:51
1994ÎÞµÐ8
гæ (³õÈëÎÄ̳)
- Ó¦Öú: 0 (Ó×¶ùÔ°)
- ½ð±Ò: 50.7
- ºì»¨: 1
- Ìû×Ó: 43
- ÔÚÏß: 15.1Сʱ
- ³æºÅ: 6877834
- ×¢²á: 2017-06-30
- רҵ: »·¾³Î¢ÉúÎïѧ
6Â¥2017-08-18 15:34:41













»Ø¸´´ËÂ¥