"...studies were cultured in URA+ selective dextrose (SD)
medium (0.7% [w/v] Difco yeast nitrogen base, 2% [w/v] glucose, 0.005%
[w/v] adenine hemisulfate, and each of L-histidine, L-tryptophan and
L-leucine at concentration of 0.003% [w/v])."
哎....我彻底晕了，大肠杆菌的克隆什么的还没搞明白呢，又得学习酵母的...
此酿酒酵母为S. cerevisiae W303(MATa leu 2-3/112 ura 3-1 trp 1-1 his 3-11/15 ade 2-1 can 1-100 mal 10 GAL SUC2)我实在看不懂，跟密码一样...
质粒p416GPD的相关性状，在文献中查到，叫URA3作为它的marker gene。不懂什么意思额。。。
回复此楼
+2/424
