²é¿´: 2593  |  »Ø¸´: 12
µ±Ç°Ö»ÏÔʾÂú×ãÖ¸¶¨Ìõ¼þµÄ»ØÌû£¬µã»÷ÕâÀï²é¿´±¾»°ÌâµÄËùÓлØÌû

happy8506

гæ (³õÈëÎÄ̳)

[½»Á÷] ¡¾ÇóÖú/½»Á÷¡¿DNase ´¦Àí RNA µÄ¾ßÌå²½Ö裨²»ÓÃÊÔ¼ÁºÐ£© ÒÑÓÐ4È˲ÎÓë

Çë½ÌÒ»ÏÂÓÃDNase ´¦Àí RNA £¬³ýÈ¥DNAµÄÏêϸ²½Ö裬×îºÃÊDz»ÓÃÊÔ¼ÁºÐµÄ¡£Ð»Ð»£¡
»Ø¸´´ËÂ¥

» ²ÂÄãϲ»¶

» ±¾Ö÷ÌâÏà¹ØÉ̼ÒÍÆ¼ö: (ÎÒÒ²ÒªÔÚÕâÀïÍÆ¹ã)

» ±¾Ö÷ÌâÏà¹Ø¼ÛÖµÌùÍÆ¼ö£¬¶ÔÄúͬÑùÓаïÖú:

ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

happy8506

гæ (³õÈëÎÄ̳)

ÒýÓûØÌû:
Originally posted by linxi8579 at 2010-05-20 18:56:23:
ÎÒÓÃµÄ TakaraµÄDNAø£¬Ð§¹ûͦºÃ£¬µ«ÊÇÒª·´Ó³Ò»¸ö°ëµ½Á½¸öСʱ£¬²»¹ýÕâÁ½ÌìÎÒµÄʵÑéÓеãÎÊÌ⣬RNAÓÐЩ½µ½â£¬DNA³ýµÄÒ²²»·Ç³£¸É¾»£¬¾ÍÊǵÚÒ»´Î×öµÃºÃ£¬ÎÒ»³ÒÉÎ񵀿»¹ÓÐÒÖÖÆ¼Á¿ÉÄÜ»îÐÔ¶¼²»ÐÐÁ˰¡£¿

ÏëÎÊϳýÈ¥DNAºóRNAµÄµçӾͼӦ¸ÃÊÇÔõÑùµÄËã×îºÃ£¿»¹ÓÐRNAºÜÈÝÒ×½µ½â£¬¸º°ËÊ®¶È±£´æÄܷŶà¾Ã£¿
9Â¥2010-05-25 10:28:41
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
²é¿´È«²¿ 13 ¸ö»Ø´ð

holyala

ľ³æ (ÖøÃûдÊÖ)

ש¼Ò

¡ï ¡ï ¡ï ¡ï
Сľ³æ(½ð±Ò+0.5):¸ø¸öºì°ü£¬Ð»Ð»»ØÌû½»Á÷
lstt09nk(½ð±Ò+3):»¶Ó­¶à¶à½»Á÷~~ 2010-05-19 18:52:29
1) È¡10¦ÌgRNAÑùÆ·ÖÃÓÚRNase¨CfreeµÄEP¹ÜÖУ¬½øÐÐDNAÏû»¯¡£Ò»°ã²ÉÓÃ20¦ÌLÌåϵ¡£
RNA                       10 ¦Ìg
DEPCË®                    n ¦ÌL
10¡ÁDNase I Buffer     2 ¦ÌL
DNase I                    2 ¦ÌL
Total volume             20 ¦ÌL

2£©37 ¡æ£¬·´Ó¦10min¡£

3£©¼ÓÈë2¦ÌL 1mM EDTA£¬75 ¡æ±äÐÔ10min£¬±ùÉÏÀäÈ´¡£

4£©²¹Ë®ÖÁ100¦ÌL£¨78¦ÌL DEPC¨CH2O£©£¬¼ÓÈë10¦ÌL 3M NaAC£¬2¦ÌLÌÇÔ­£¬»ìÔȺó¼ÓÈë2.5±¶Ìå»ýµÄÎÞË®ÒÒ´¼£¬ÔٴλìÔÈ£¬-80 ¡æ¾²ÖÃ30min³ÁµíRNA£¬13000 rpm 4 ¡æÀëÐÄ20min¡£70%ÒÒ´¼£¨DEPC¨CH2OÅäÖÆ£©Ï´µÓ²¢13000 rpmÀëÐÄ5min¡£ÉÔ΢ÁÀ¸ÉºóÓÃDEPC¨CË®Èܽâ³Áµí¡£
2Â¥2010-05-19 17:25:04
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

zhwenxing

ľ³æ (ÖøÃûдÊÖ)

Œ­É­Ïx

¡ï ¡ï ¡ï ¡ï
Сľ³æ(½ð±Ò+0.5):¸ø¸öºì°ü£¬Ð»Ð»»ØÌû½»Á÷
lstt09nk(½ð±Ò+3):»¶Ó­¶à¶à½»Á÷~~ 2010-05-19 18:51:58
1¡¢Ê×ÏÈÒª´ò¿ªRNA µÄ¶þ¼¶½á¹¹¡£70-72¡æ10minºóÁ¢¿Ì±ùÀä¡£
2¡¢ÌåϵÓдóÓÐС¸ù¾Ý˵Ã÷ÊéÀ´¾Í¿ÉÒÔÁË¡£
3¡¢Ìرð×¢ÒâDNAseµÄŨ¶È£¡Ò²¾ÍÊǵ¥Î»Êý£¬Ò»°ãÏ¡ÊͺóʹÓã¬1/1000ÓÐʱºò¾Í×ã¹»À²¡£
4¡¢¹ýÖù×Ó·¨»òÕß³Áµí·¨»ØÊÕRNA£¬¿Ï¶¨»áÓв»ÉÙËðʧµÄ~¶à×¼±¸µãRNA¹þ~

×£¸£ºÃÔË£¡
3Â¥2010-05-19 17:49:12
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

happy8506

гæ (³õÈëÎÄ̳)

ÒýÓûØÌû:
Originally posted by holyala at 2010-05-19 17:25:04:
1) È¡10¦ÌgRNAÑùÆ·ÖÃÓÚRNase¨CfreeµÄEP¹ÜÖУ¬½øÐÐDNAÏû»¯¡£Ò»°ã²ÉÓÃ20¦ÌLÌåϵ¡£
RNA                       10 ¦Ìg
DEPCË®                    n ¦ÌL
10¡ÁDNase I Buffer     2 ¦ÌL
DNase I       ...

лл£¬¿ÉÊÇÒ»¶¨Òª¼ÓÌÇÔ­Âð£¿
4Â¥2010-05-19 18:15:03
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[˶²©¼ÒÔ°] ÊÛSCIÒ»ÇøÎÄÕ£¬ÎÒ:8.O.551.O.5.4,¿ÆÄ¿È«,¿ÉÙ¤¼± +3 0XLacIJUOj8D 2026-08-24 6/300 2026-08-25 00:11 by BZKMTicpDhFj
[»ù½ðÉêÇë] ÈËÆø²»ÐÐÁË +10 fansofjerry 2026-08-21 10/500 2026-08-24 21:03 by zhanghaozhu
[»ù½ðÉêÇë] ÎÒÃæÉÏÍêµ°ÁË +9 ÇÒÌý»¢Ð¥ 2026-08-20 10/500 2026-08-24 20:08 by ¿àº®À´Ïã
[»ù½ðÉêÇë] filecode£¬4¸öjtjcÁË +14 ziyangfang 2026-08-19 17/850 2026-08-24 18:37 by ¹þ¹þ¸ò£¿
[»ù½ðÉêÇë] 2026¹ú×ÔÈ»º¯ÆÀ·Ñµ½ÕË +17 ÑòÑü°å 2026-08-21 19/950 2026-08-24 16:52 by iaeyuan
[»ù½ðÉêÇë] ½ñÈÕ²»·Å°ñ£¿Íø´«¹ú×ÔȻԤ¼Æ 8 Ô 27 Èտɲé½á¹û +17 ҽѧÀÏÄк¢ 2026-08-20 21/1050 2026-08-24 14:21 by refreshing11
[»ù½ðÉêÇë] ¹À¼ÆÊÇÖÜËÄ +4 archvillain 2026-08-18 4/200 2026-08-24 13:53 by zzuzxg
[»ù½ðÉêÇë] ·¶½øÖоÙÒ»ÎĵÄÖÐÐÄ˼Ïë +6 Ñ׻ƹóëÐ 2026-08-22 7/350 2026-08-24 11:58 by 6543yes
[»ù½ðÉêÇë] ÅóÓÑȦ¿´µ½µÄ +7 wangzilk 2026-08-18 9/450 2026-08-24 10:50 by cmrandy
[»ù½ðÉêÇë] ¿ÆÑй¶ùÌ«ÄÑÁË +18 ÎÒ4´ó°×²Ë 2026-08-20 19/950 2026-08-24 09:47 by ¿­¶÷¹ãÊ¢´ß»¯·ÖÎ
[»ù½ðÉêÇë] 2026ÄêµÄ¹ú¼ÒÉç¿Æ»ù½ðÏîĿͨѶÆÀÉóµÄйæÔòÓëж¯Ïò¡¢ÐÂÌôÕ½ +4 process2012 2026-08-23 5/250 2026-08-23 19:58 by jurkat.1640
[»ù½ðÉêÇë] ½ñÌì·Å°ñÂ𣿠+15 ²¼²¼ºÍÒ»¶þ 2026-08-19 16/800 2026-08-23 09:55 by ÕÅ´ºÉú
[»ù½ðÉêÇë] Ö»ÓÐÿÄêÕâÖÖʱºòÀ´¹ä¹äСľ³æ +24 yaoyewhu2008 2026-08-20 26/1300 2026-08-22 17:43 by kammury
[»ù½ðÉêÇë] ʱ¼ä´Á½ñÌ죬20ºÅ±äÁË +5 archvillain 2026-08-20 5/250 2026-08-22 06:12 by hui_daxiao
[»ù½ðÉêÇë] ʱ¼ä´ÁÓÖ±äÁË +13 wuchongjun 2026-08-20 19/950 2026-08-21 17:21 by ×Ïɼ´¼
[»ù½ðÉêÇë] Ó¦¸ÃÊÇÏÂÖÜÈý26ÈÕ¹«²¼Á˰ɣ¿ +4 ¹þ¹þ¸ò£¿ 2026-08-21 4/200 2026-08-21 10:58 by Vivilian
[»ù½ðÉêÇë] ½ñÌì·Å°ñûϷÁ衃 +9 yuleib84 2026-08-19 11/550 2026-08-21 10:06 by gltch
[»ù½ðÉêÇë] »ù½ð°¡»ù½ð +4 longfie172 2026-08-20 4/200 2026-08-21 08:58 by mark mao
[»ù½ðÉêÇë] ÖØÒªÏûÏ¢£¬ÖÐÎçϵͳÔÚά»¤ +11 yuleib84 2026-08-18 12/600 2026-08-20 11:09 by xskun
[»ù½ðÉêÇë] Ã÷Ìì·Å°ñ£¿ +5 Shxjjxjkx 2026-08-18 5/250 2026-08-18 18:14 by -´ó´ó´ó´ó´ó-
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û