5楼: Originally posted by yuy111 at 2016-09-07 01:14:56
Agrose must be fully solved and got a clear solution after heating, pour it before agrose solution cold , hotter is better than colder . TAE buffer to run the gel should be fresh made, not reusable.  ...

2楼: Originally posted by 卡维斯 at 2016-04-14 17:11:53
1X TAE和相应的琼脂糖配置。
先将其一次性煮沸，中间可以轻轻摇晃两三下--->再煮沸三次，每次沸腾即可，摇匀后再加热--->透彻没不溶物即可，然后可冲凉注意摇晃防止局部凝固，温度以手背可忍受为止，加入相应 ...