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ÎÒÃÇʵÑéÊÒÓõÄÊǸÄÁ¼CTAB·¨£¬²Ù×÷ʱ¼ä³¤£¬¿ÉÊÇЧ¹ûÌØ±ðºÃ£¬ÎÒÃÇÒ»°ã¶¼ÌáÀϱ걾µÄ£¬ÏñÂ¥Ö÷µÄµÄÐÂÏÊҶƬÓôËÖÖ·½·¨Ó¦¸Ã¸üûÎÊÌâ°É
3Â¥2016-01-04 09:57:30
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You could try this protocol, which is very useful for plant total DNA extraction.

I hope you could improve the CTAB method for Soil microorganism total DNA extraction by drawing lessons from this protocol.

3% CTAB DNA extracion protocol
(modified from Zeng et al. 2002, ACta Botanica Sinica 44; 694-697)

1. washing out polysaccharides with CTAB-free buffer from homogenate.
2. increasing the percentage of CTAB in extraction medium.
3. using high concentration salts pior to DNA precipitation with isopanol.

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  • ¸½¼þ 1 : 3%_CTAB_DNA_Extraction_Protocol.pdf
  • 2016-01-05 21:39:19, 50.16 K
  • ¸½¼þ 2 : Preparation_of_Total_DNA_from_Recalcitrant_Plant_Taxa.pdf
  • 2016-01-05 21:39:19, 201.62 K
5Â¥2016-01-05 21:39:57
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