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jyqx2008

木虫 (小有名气)

[求助] E.coli numbering已有1人参与

亲们,有谁知道E.coli numbering是什么意思,在看放线菌中的MAR1,说这类放线菌的E.coli numbering的Nucleotide position(16S rRNA)的有些碱基比较特殊,可是看测序结果时没有发现这些位置碱基的特殊性,E.coli numbering是不是不是提交的序列中的信息?求助,非常感谢。
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xuyunjian

铁虫 (小有名气)

【答案】应助回帖

感谢参与,应助指数 +1
看下面
区别Photorhabdus和Xenorhabdus两个属的DNA碱基差异在383和503的位置(E.coli numbering),所有的Xenorhabdus为G和A,而所有的Photorhabdus为A和C。
http://cdmd.cnki.com.cn/Article/CDMD-11920-2004091330.htm
  Figure 5. Structure of conserved residues within the 23S rRNA active site from the H.marismortui 50S subunit crystal structure (2). The A2450+C2063 wobble pair (E.coli numbering) is shown in orange, A2451 is shown in blue, G2061 and G2447 are shown in green and C2501 is in yellow. The N1 of A2451 that reacts with DMS is shown as an enlarged sphere. The hydrogen bonds between A2450+C2063 that are likely to be disrupted by mutation to a G?U pair are shown in red. This includes interactions within the A2450+C2063-C2501 triple, as well as the G2061-A2451-G2447 triple stacked above it.
原文链接:http://www.100md.com/html/DirDu/2007/02/17/37/12/79.htm
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2楼2014-07-20 23:14:38
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