24小时热门版块排行榜    

查看: 2189  |  回复: 2

lixin601

木虫 (正式写手)

[求助] 序列中有多个终止密码子,基因序列提交不到到genbank,请看一下genbank的回信

最近获得了一些微生物的KS基因序列,想提交到genbank,但提交了几次都不合格。不知道什么原因,还有别的办法吗?
We have noticed that some or all of the sequences contain internal stop
codons and/or reading frame shifts (insertions/deletions based on BLAST
similarity search results and/or an alignment).

This generally indicates problems such as:

  - errors in the nucleotide sequence
  - sequences have not been trimmed of low quality
  - problems with feature annotation which may be due to:
        translation in the wrong reading frame or on the wrong strand
        translation does not have the correct exon spans
        translation includes noncoding sequence, such as introns or UTR's
          
If the sequences and/or annotation are incorrect, please resubmit
with the corrected files. If the information is correct, please explain
the presence of the internal stop codons and/or frame shifts.

If you are unable to determine the correct sequence and/or annotation
for your sequence(s), they can still be processed and the following
comment will be added to your records:

COMMENT  GenBank staff is unable to verify sequence and/or annotation
         provided by the submitter.
         
In addition, the record(s) will indicate that the data are unverified and
your sequence(s) will not be included in NCBI BLAST databases.
回复此楼

» 猜你喜欢

» 本主题相关价值贴推荐,对您同样有帮助:

已阅   关注TA 给TA发消息 送TA红花 TA的回帖

bianyu

铜虫 (正式写手)

不懂这个呢,不过想请教楼主ks基因的相关问题,不知楼主的KS基因有什么作用啊?是跟什么相关的呢?我看过有的跟脂肪酸合成相关~
2楼2014-03-20 11:32:37
已阅   关注TA 给TA发消息 送TA红花 TA的回帖

lixin601

木虫 (正式写手)

引用回帖:
2楼: Originally posted by bianyu at 2014-03-20 11:32:37
不懂这个呢,不过想请教楼主ks基因的相关问题,不知楼主的KS基因有什么作用啊?是跟什么相关的呢?我看过有的跟脂肪酸合成相关~

酮基合酶基因,主要与抗生素类化合物生物合成有关,不知道你们做的是否与我们相似,请帮忙看一下
谢谢
3楼2014-03-20 12:43:52
已阅   关注TA 给TA发消息 送TA红花 TA的回帖
相关版块跳转 我要订阅楼主 lixin601 的主题更新
最具人气热帖推荐 [查看全部] 作者 回/看 最后发表
[考研] 291分工科求调剂 +3 science饿饿 2026-03-01 3/150 2026-03-01 07:49 by ms629
[考研] 272求调剂 +4 田智友 2026-02-28 4/200 2026-03-01 06:43 by 刘兵
[考研] 285求调剂 +6 满头大汗的学生 2026-02-28 6/300 2026-03-01 06:29 by Trying]
[基金申请] 面上模板改不了页边距吧? +5 ieewxg 2026-02-25 6/300 2026-03-01 00:10 by addressing
[考研] 307求调剂 +4 73372112 2026-02-28 6/300 2026-03-01 00:04 by ll247
[考研] 304求调剂 +3 52hz~~ 2026-02-28 5/250 2026-03-01 00:00 by 52hz~~
[考研] 化工专硕348,一志愿985求调剂 +4 弗格个 2026-02-28 6/300 2026-02-28 22:00 by wang_dand
[考博] 26申博 +4 想申博! 2026-02-26 4/200 2026-02-28 21:37 by limorning
[考研] 材料类求调剂 +6 wana_kiko 2026-02-28 6/300 2026-02-28 21:20 by gaoxiaoniuma
[考研] 求调剂 +4 repeatt?t 2026-02-28 4/200 2026-02-28 21:16 by gaoxiaoniuma
[考研] 284求调剂 +4 天下熯 2026-02-28 4/200 2026-02-28 21:13 by gaoxiaoniuma
[考研] 085600材料工程一志愿中科大总分312求调剂 +8 吃宵夜1 2026-02-28 10/500 2026-02-28 20:27 by L135790
[考研] 298求调剂 +8 人间唯你是清欢 2026-02-28 11/550 2026-02-28 20:26 by L135790
[考研] 0856材料求调剂 +10 hyf hyf hyf 2026-02-28 11/550 2026-02-28 18:50 by 无际的草原
[考博] 博士自荐 +3 kkluvs 2026-02-28 3/150 2026-02-28 16:59 by StarAura
[考研] 265分求调剂不调专业和学校有行学上就 +4 礼堂丁真258 2026-02-28 6/300 2026-02-28 16:18 by 求调剂zz
[考研] 寻找调剂 +3 LYidhsjabdj 2026-02-28 3/150 2026-02-28 12:59 by miniwendy
[考研] 304求调剂 +5 曼殊2266 2026-02-28 6/300 2026-02-28 12:44 by 迷糊CCPs
[硕博家园] 博士自荐 +6 科研狗111 2026-02-26 9/450 2026-02-28 12:32 by seaskyy
[基金申请] 面上可以超过30页吧? +12 阿拉贡aragon 2026-02-22 13/650 2026-02-26 22:09 by Hahaxia
信息提示
请填处理意见