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lixin601

木虫 (正式写手)

[求助] 序列中有多个终止密码子,基因序列提交不到到genbank,请看一下genbank的回信

最近获得了一些微生物的KS基因序列,想提交到genbank,但提交了几次都不合格。不知道什么原因,还有别的办法吗?
We have noticed that some or all of the sequences contain internal stop
codons and/or reading frame shifts (insertions/deletions based on BLAST
similarity search results and/or an alignment).

This generally indicates problems such as:

  - errors in the nucleotide sequence
  - sequences have not been trimmed of low quality
  - problems with feature annotation which may be due to:
        translation in the wrong reading frame or on the wrong strand
        translation does not have the correct exon spans
        translation includes noncoding sequence, such as introns or UTR's
          
If the sequences and/or annotation are incorrect, please resubmit
with the corrected files. If the information is correct, please explain
the presence of the internal stop codons and/or frame shifts.

If you are unable to determine the correct sequence and/or annotation
for your sequence(s), they can still be processed and the following
comment will be added to your records:

COMMENT  GenBank staff is unable to verify sequence and/or annotation
         provided by the submitter.
         
In addition, the record(s) will indicate that the data are unverified and
your sequence(s) will not be included in NCBI BLAST databases.
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bianyu

铜虫 (正式写手)

不懂这个呢,不过想请教楼主ks基因的相关问题,不知楼主的KS基因有什么作用啊?是跟什么相关的呢?我看过有的跟脂肪酸合成相关~
2楼2014-03-20 11:32:37
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lixin601

木虫 (正式写手)

引用回帖:
2楼: Originally posted by bianyu at 2014-03-20 11:32:37
不懂这个呢,不过想请教楼主ks基因的相关问题,不知楼主的KS基因有什么作用啊?是跟什么相关的呢?我看过有的跟脂肪酸合成相关~

酮基合酶基因,主要与抗生素类化合物生物合成有关,不知道你们做的是否与我们相似,请帮忙看一下
谢谢
3楼2014-03-20 12:43:52
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