酶活定义: One unit ofenzyme activity was defined as the amount that catalyzed the oxidation of 1 μmol NADPH/NADH per minute。
已知双倒数法拟合出的方程式为: y = 0.0141x + 0.0042 ,已知蛋白浓度为3mg/ml, 反应体系为1 ml,加入蛋白量10ul, NAD(P)H的e=6.2/mM cm,340 nm,比色皿是1cm的,请问怎么求Kcat (单位:s-1),谢谢!