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Reviewer #2: The authors did address most concerns of the reviewer. However, there is one technical issue which has to be clarified.

1. In the MM section, the authors state that "To ensure that the samples were free of genomic DNA, RNA samples were subjected to a reverse transcription in the absence of the reverse transcriptase. Our results didn't find any genomic DNA contamination." The authors are correct to assume that if there is any contamination with genomic DNA, then there should be amplification in the subsequent PCR. HOWEVER, in case the primer is an exon-intron spanning oligonucleotide and the amplified intron is too long, than this genomic contamination band will not be visible in a 1% agarose gel. I strongly suggest performing another control experiment with contaminated RNA to ensure that the assay performed is sufficient to detect genomic DNA contamination.

3. Which strategy was followed to evaluate real-time PCR experiments?
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The doer of good becomes good, the doer of evil becomes evil.
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2Â¥: Originally posted by fuyuandj86 at 2013-09-27 11:21:50
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3Â¥: Originally posted by niil at 2013-09-27 14:00:30
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