| 查看: 2224 | 回复: 3 | ||
[求助]
有没有人用过去DNA甲基化的酶？
|
| 有没有可以在体外进行DNA脱甲基化的酶？有人用过么？ |
回复此楼» 猜你喜欢
爱思维尔旗下LWT投稿
已经有9人回复
-
替拉扎明为缺氧选择性细胞毒药物研究提供新思路
已经有0人回复
-
化学工程及工业化学论文润色/翻译怎么收费?
已经有115人回复
-
拉坦前列腺素(Latanoprost)：前列腺素F2α衍生物如何成为青光眼一线用药
已经有0人回复
-
卟啉家族中的明星分子：MESO-四(3,5-二溴-4-羟基苯基)卟啉的性质与制备
已经有1人回复
-
宠物寄生虫防治：氟雷拉纳阻断GABA受体，持效12周驱杀跳蚤蜱虫
已经有0人回复
-
为呼吸打开通道：依伐卡托的科学之旅
已经有1人回复
-
Dordaviprone（ONC201）：小分子药物在中线胶质瘤中的应用
已经有0人回复
-
依喜替康：新型喜树碱衍生物的研究进展
已经有1人回复
-
膀胱癌靶向治疗新选择：厄达替尼作用机制与耐药研究进展
已经有0人回复
-
从水母到实验室：腔肠素的发现历程与生物发光奥秘
已经有0人回复
» 本主题相关价值贴推荐，对您同样有帮助:
- 请问提出rna来还有三条带，为什么dna酶消化检测后后，就拖带了？
已经有10人回复
- 反转录前 DNA 酶消化
已经有10人回复
- 质粒酶切切不动，质粒在宿主中繁衍酶切位点会突变么？
已经有8人回复
- 求助MSP凝胶电泳该如何选择
已经有16人回复
- DNA甲基化检测技术
已经有10人回复
- 大家有没有做过DNA甲基化的实验?
已经有9人回复
- 使用亚硫酸氢钠测序法测试基因CpG甲基化
已经有15人回复
- 有没有人做太湖底泥微生物DNA提取的啊
已经有8人回复
- 【求助/交流】定点突变PCR后加DPN1酶的作用
已经有4人回复
- 有关感受态细胞DH5a的基因型
已经有6人回复
ADTechnology 
捐助贵宾 (著名写手)
表观遗传学专业供应商
- 应助: 12 (小学生)
- 金币: 12813.7
- 散金: 1762
- 红花: 1
- 帖子: 2671
- 在线: 51.6小时
- 虫号: 1787705
- 注册: 2012-04-30
- 专业: 人类遗传学
【答案】应助回帖
★ ★ ★ ★ ★
liuxiuaza: 金币+5 2013-06-04 12:52:32
liuxiuaza: 金币+5 2013-06-04 12:52:32
|
超纯DNA去甲基化活性/抑制分析试剂盒 货号：A-P-3008 EpiQuik DNA Demethylase Activity/Inhibition Assay Ultra Kit (48 assays) 背景资料：DNA demethylation is necessary for the epigenentic reprogramming of genes and is also directly involved in many important disease mechanisms such as tumor progression. Demethylation of DNA can either be passive or active, or a combination of both. Passive DNA demethylation usually takes place on newly synthesized DNA strands via DNMT1 during replication rounds. Active DNA demethylation mainly occurs by the removal of 5-methylcytosine through further modified cytosine bases which have been converted by TET enzyme-mediated oxidation. These oxidation products have been shown to be repaired by TDG, a glycosylase which is involved in base excision repair, or directly converted to cytosine by DNMT3A/ DNMT3B in oxidative states. It is proposed that DNA demethylation could also be initiated by deamination of 5-mC through candidate deaminases including AID and APOBEC1, which convert 5-mC to thymine. The resulting thymine could be repaired by BER initiated by a T-G mismatch glycosylase such as MBD4 or TDG. In addition, the 5-mC base can be directly removed in plants by the DME/ROS1 family of 5-mC DNA glycosylases, resulting in an abasic site that is repaired by the BER process. 产品描述：The EpiQuik™ DNA Demethylase Activity/Inhibition Assay Ultra Kit is a complete set of essential components which enables an experimenter to measure total DNA demethylase activity/inhibition. The kit can be used with nuclear extracts from a broad range of species such as mammals and plants, in a variety of forms including, but not limited to, cultured cells and fresh tissues. 产品特点： 1·Colorimetric assay with easy-to-follow steps for convenience and speed. The entire procedure can be completed within 4 hours； 2·Safe and innovative colorimetric assay without the need for radioactivity, extraction, or chromatography； 3·An ultra-sensitive detection limit, as low as 1 µg of nuclear extract, which is five times more sensitive than the predecessor kit； 4·96 strip-well microplate format allows for either low or high throughput analysis。 不知道您是具体想做什么实验呢？  |
2楼2013-06-01 17:07:16
3楼2018-04-28 16:30:44
4楼2018-04-29 09:26:45
5