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| Since we found a significant change in growth of suspension cells (Fig. 3). The cell number was in a lower level before 4 h, but then rapidly raised with high budding rate and low mortality rate. When fermented for 16 h, the cell number achieved a maximum value of about £¿ million/mL, which was closed to industrial level. However, increased ethanol exhibited a strong inhibition on yeast cells in the later period of fermentation, which led to a low budding rate and high mortality rate, thereby showing a decreased tendency in cell number. In addition, after 11 batches, a layer of enriched cells was observed in some place of carrier surface with the naked eye, which was not in consistent with Choi et al. who reported that it was difficult to accumulate cells through repeated batch processes. This result indicated that cell immobilization can promote cell growth and not seriously affected by sludge and suspended solid in the cassava meal medium. |
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Ìú¸Ëľ³æ (ÖøÃûдÊÖ)
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...£¬since we found a significant change in growth of suspension cells (Fig. 3). From the figure, we can see that the cell number is in a lower level at the first 4 hours of the fermentation. But, subsequently, the number raises rapidly due to the high budding rate and low mortality rate. Finally, the number reaches the maximum value #/mL, which approaches the level of industrial production, at the time 16 hours after the beginning of the fermentation. However, this is not a real ending. As time goes futher, the number trends to decrease because of the increase of ethanol. The rise of ethanol has a strong inhibition on yeast cells, and therefore results in a low budding rate and high mortality rate. In addition, after 11 batches, a layer of enriched cells is visible in some place of carrier surface. This is not in consistent with the results of Choi et al. [] which suggest(show, reveal, indicate) that it was difficult to accumulate cells through repeated batch processes. Our results suggest that cell immobilization can promote cell growth. The results also indicate that the cell growth is not seriously affected by sludge and suspended solid in the cassava meal medium. |
6Â¥2013-04-18 01:01:15
LT_FU
Ìú¸Ëľ³æ (ÖøÃûдÊÖ)
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2Â¥2013-04-17 20:42:34
liuqg1987
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3Â¥2013-04-17 21:12:07
LT_FU
Ìú¸Ëľ³æ (ÖøÃûдÊÖ)
- Ó¦Öú: 110 (¸ßÖÐÉú)
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4Â¥2013-04-17 21:13:38













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