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zybpxy

铁杆木虫 (著名写手)

致斋大人

应助: 506 (博士)
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金币: 5395.7
散金: 2020
红花: 54
沙发: 1
帖子: 2415
在线: 341.3小时
虫号: 2019461
注册: 2012-09-21
性别: GG
专业: 食品科学基础

[求助] 关于脂肪酸GC及GC-MS定性的问题已有1人参与

待分析样品：淡水鱼肌肉脂肪酸组成；
文献中采用FID检测器进行检测，并用了sigma的混标进行定性。请问如何得知混标中37种脂肪酸甲酯的出峰时间呢？
小弟的柱子是DB-5
下附文献及标品网址
Approximately 50–100 mg lipids were then used for lipid class determinations by gas chromatography. Fatty acid methyl esters (FAME) were prepared by transesterification with borontrifluoride (BF3) in methanol at 70 °C. FAME was run on an Shimadzu GC-2010 gas chromatograph equipped with aflame ionization detector, a fused silica capillary column (30 m × 0.25 mm ID × 0.3 μm film, FFAP, Dalian Institute of Chemical Physics Chinese Academy of Sciences) and GC solution software. Hydrogen was used as the carrier gas (flow velocity: 1.0 ml min− 1). The injector temperature was adjusted to 260 °C, and the column temperature was increased from 160 °C to 230 °C at a rate of 2.0 °C min− 1 and then held at 230 °C for 15 min. The detector was maintained at 230 °C. The identifications of fatty acids were based on comparisons of retention times with known FAME standard mixtures (a 37-component FAME mix and menhaden oil, Sigma, Chemical Co, USA).

标品网址：http://www.sigmaaldrich.com/cata ... ng=zh®ion=CN

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