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suyuhao

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[资源] 蛋白冻融的基本策略！！不容易找到的英文教材里的部分内容，分享给大家

Brief Guidelines for Freezing and Thawing Protein Samples

Disclaimer: These guidelines are intended for use in general with protein solutions, but the stability of individual proteins varies widely. The investigator must determine the proper storage and freeze-thaw conditions for each protein.

For proteins purchased from PBL InterferonSource, refer first to the data sheet . In the absence of written guidance, contact technical service (at 1 877-PBL-8881 or + 1 732-777-9123 for recommendations on handling of specific proteins.

It is usually best to work with protein solutions on wet ice. The low temperature will slow inactivation of the protein.

Never vigorously agitate a protein sample. The preferred method of mixing protein solutions is to gently mix with a micropipettor using a polypropylene tip. (Do not use larger pipettors with polystyrene pipettes.)

An alternative for larger solutions is gentle inversion in a 15 to 50 ml capped polypropylene tube.

For volumes over 50 ml, mixing using a stir bar is acceptable, but not too vigorously. Never introduce foam or air bubbles ?these denature proteins.

Do not use glass or polystyrene containers or pipettes for antibodies or interferons unless these proteins are diluted in serum or albumin-containing media.

Freeze/Thaw of proteins. Usually a quick freeze and a quick thaw are the best methods for retaining protein activities. Rapid freezing and thawing prevent phase partitioning of the salts or protein. If a protein solution is found to be cloudy upon thawing, the bioactivities of the protein are likely to have been adversely affected.

Freezing method
Preferred:
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附件 1 : General Protein Handling Guideline 之冻融蛋白的基本策略.pdf

2011-12-05 11:38:53, 52.67 K

1楼2011-12-05 11:40:11

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abigale01989

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对于我这种门外汉来说，很有用，谢谢！

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35楼2015-11-04 16:14:23

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jinhx872楼

2011-12-05 13:10 回复

五星好评顶一下，感谢分享！

goldenleaf3楼

2011-12-05 13:50 回复

五星好评顶一下，感谢分享！

sph1504楼

2011-12-05 14:08 回复

五星好评顶一下，感谢分享！

smithgao5楼

2011-12-05 14:42 回复

一般

引用回帖:

1楼: Originally posted by suyuhao at 2011-12-05 11:40:11: Brief Guidelines for Freezing and Thawing Protein Samples Disclaimer: These guidelines are intended for use in general with protein solutions, but the stability of individual proteins varies wi ...

yyk816楼

2011-12-05 16:48 回复

五星好评顶一下，感谢分享！

zhenzh8577楼

2011-12-05 18:23 回复

五星好评顶一下，感谢分享！

langya258楼

2011-12-06 03:35 回复

五星好评顶一下，感谢分享！

yankelu9楼

2011-12-06 07:14 回复

五星好评顶一下，感谢分享！