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How was the quality of your RNA extracted?

I think your PCR templates were rich in poly/oligosaccharides, polyphenols and other unknown sticky compounds that bind or co-precipitae with DNA and form a viscous complex. Such DNA sample is usually neither suitable for PCR amplification nor even possible to load into the electrophoresis well.
3Â¥2016-01-11 10:59:15
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