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Modern bonded RP-materials Improving the resolution achieved through ultralow dispersion systems, smaller particles and welldesigned columns were stopped by pressure increase. The new millennium has brought important achievements in this field, namely the core-type spherical particles, allowing preparation of more uniform column beds with much more narrow size distribution ofapproximately 6% relative standard deviation delivering high efficiency and high separation speed at a much lower pressure [8]. The minimum in van Deemter relationship, illustrating the theoretical plate height relation to flow velocity has almost disappeared. It means that velocity increases can maintain efficiency at an almost constant level. |
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