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µÚÒ»´ÎͶӢÎÄÎÄÕ£¬Í¶¸åºóÒ»¸öÔÂÊÕµ½±à¼­µÄ»Ø¸´ÁË£¬½¨Òé´óÐÞ¡£Ò»¹²ÕÒÁËÁ½¸öÉó¸åÈË£¬µÚÒ»¸öÒâ¼û±È½Ï¼âÈñ²»½¨Òé½ÓÊÕ£»µÚ¶þ¸ö±È½ÏniceһЩ£¬ÌáÁË6¸öÎÊÌ⣬µ«ÊǶ¼ÊDZȽÏpositiveµÄ£¬Ò»Ð©½¨ÒéʲôµÄ¡£ Áí¸½ÉÏreviewer1µÄÒâ¼û´ó¼Ò°ïæ·ÖÎöһϸÃÈçºÎ»Ø¸´£¬·Ç³£¸ÐлÁË¡£

Reviewer #1: General comments

This study investigated ¡°ÎÄÕ±êÌâ...." ¡£As the results, hPDLCs conditioned by¡± Ò»ÖÖÎïÖÊ¡° demonstrated a significant increase in proliferation and also showed an increased formation of mineralized matrix nodules and increased ALP activity with the attendant expression of Runx2. Based on these findings, the authors suggested that ¡±Ò»ÖÖÎïÖÊ¡° induces proliferation and osteogenic differentiation of hPDLCs, indicating that¡±Ò»ÖÖÎïÖÊ¡° may be applicable as a novel autologous graft in periodontal tissue engineering regeneration. The aims of this study are clear and the results obtained are interesting. However, the current findings appeared to quite preliminary data. Without the data showing the possible mechanisms by which¡±Ò»ÖÖÎïÖÊ¡° stimulated proliferation and osteoblast differentiation of hPDLCs, it can't be further considerable for acceptance.

Specific comments
First of all, there was no a study to verify the mechanisms involved in the ¡±Ò»ÖÖÎïÖÊ¡°-mediated increases in proliferation and differentiation of the cells. It is strongly recommended to show why the blood samples did so in the hPDLCs. Especially, to end this, the authors have to try to check cell cycle progression using flow cytometer and then to analyze what are the regulatory factors such as CDKs and/or cyclins are involved therein by Western blot analysis. In addition, the authors showed the expression pattern of Runx2 as an osteogenic marker. Of course, this factor might be one of the most important osteogenic factors. However, it will be needed to show that which of the signaling molecules are closely associated with the induction of Runx2 as a second messenger by treating the cells with the blood samples. With these additional experiments, the authors are also capable of explaining the reason why the ¡±Ò»ÖÖÎïÖÊ¡° among the samples showed the most prominent activities.

Minor comments
1.        The figures 2 and 3 are not necessary in this manuscript, rather it is recommended to show as a supplemental figure.
2.        Please check carefully misspelling (ex, HPDLCs -> hPDLCs) and grammatical errors.
3.        The length of several sentences shown in the highlights appeared to be over a general limitation. Please check this with consideration of the Journal Policy.

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