我用的是avidin-GOx做的探针,但是审稿人却让我看一看GOx是否有非特异性吸附。哎,不知道该怎么办,发愁ing
The authors could use a few controls demonstrating the sensor’s performance if certain modifications aren’t provided. For instance, non-specific binding is always an issue with these types of bead assays. Will GOx nonspontaneously adsorb onto the beads – i.e. without the avidin coupling?