24小时热门版块排行榜    

CyRhmU.jpeg
查看: 432  |  回复: 2

leisonn

银虫 (小有名气)

[求助] 审稿意见看不懂,请高手指点,多谢!

之前投了篇文章,其中有一小块内容做了花瓣类黄酮含量的测定,我是按照其他文献采用ALCL3比色法做的,原文如下:20 mg of petal tissues was extracted in 10 ml of 80% methanol overnight on a shaker. The extract was cleared by centrifugation at 12,000 × g for 30 min, then 4.5 ml of the supernatant was recovered, to which 500 μl of 10% AlCl3 in methanol was added to a final concentration of 1%. After 10 min, the absorbance at 420 nm was measured, which were also normalized to the petal fresh weight.   
现在审稿意见中对该方法提出了质疑,由于本人对这方面不是很了解,不知如何回答,特来请教各位高手,请帮我看看哈~~~意见如下:
Authors used total flavonoids as the meaning of flavonols and flavones. However, anthocyanins are also contained in flavonoids. The quantitative determination using AlCl3 regent doesn't indicate total flavonoid concentration containing anthocyanins.
多谢!!
回复此楼

» 猜你喜欢

» 本主题相关价值贴推荐,对您同样有帮助:

已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

leisonn

银虫 (小有名气)

请高手帮帮忙啊
2楼2013-09-25 12:03:43
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

linweikang

至尊木虫 (知名作家)

【答案】应助回帖

感谢参与,应助指数 +1
就是说你的论文存在专业性错误。你用总黄酮替代黄酮醇和黄酮,但是你忽略了黄酮里面也存在花青素。那么,你用三氯化铝来定量分析表征含有花青素的黄酮浓度是不正确的。
3楼2013-09-26 13:23:01
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
相关版块跳转 我要订阅楼主 leisonn 的主题更新
信息提示
请填处理意见