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gaojie1402

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[求助] 基因提交问题

我向NCBI提交基因遇到一些问题求解答
他是这样回复我的
This generally indicates problems such as:

- errors in the nucleotide sequence
- sequences have not been trimmed of low quality or vector
- problems with feature annotation which may be due to:
translation in the wrong reading frame or on the wrong strand
translation does not have the correct exon spans
translation includes noncoding sequence, such as introns or UTR's
   
If the sequences and/or annotation are incorrect, please resubmit
with the corrected files. If the information is correct, please explain
the presence of the internal stop codons and/or frame shifts.
  我确定自己的序列没有错  求大侠们帮我解决 我很急  看到第一时间帮忙  谢谢!
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