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[求助]
AGL6基因与VP16转录激活
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有做拟南芥的同学吗？请教个问题啊： 说AGL6基因与VP16这个转录激活子结合后转入到拟南芥中，结果导致了拟南芥长出了原本没有的苞片组织，作者就说AGL6基因具有抑制苞片组织生长的作用，请问该怎么理解？谢谢 原文：Ectopic bracts and abnormal flowers in gAGL6::VP16 plants To tease apart the confounding problems of misexpression, we expressed a fusion of AGL6 to the strong transcriptional activation domain from the viral transcription factor VP16 (Triezenberg et al., 1988; Cress and Triezenberg, 1991) in the context of a 5.2 kb genomic fragment that included 2.6 and 0.8 kb of sequences upstream of the start codon and downstream of the stop codon, respectively. The resulting gAGL6::VP16 construct contains all important regulatory elements for AGL6 expression located in both the promoter region and the large first intron, which have been identified recently (Schauer et al., 2009), suggesting identical expression pattern of AGL6 in both wild-type and gAGL6::VP16 plants. In contrast to Pro35S:AGL6 and Pro35S:AGL6::EAR plants, gAGL6::VP16 plants had normal rosette leaves (Figure 4a). In addition, flowering time was normal (Table 1). Striking phenotypes were seen in the inflorescences of gAGL6::VP16 plants. Most flowers had ectopic organs on the pedicel (Figure 4b,c). Usually the first flower, designated as f1 in Figure 4(j), was normal. The second flower already showed signs of abnormal development, with a filamentous structure on the pedicel. Subsequently, the ectopic organs became more fully developed, with sepaloid/bract/leaf-like identity in the next two to three flowers. Bract-like features included pointed tips, lack of petioles and delayed senescence compared with sepals (Dinneny et al., 2004). At the insertion point of the bract, there was a kink in the pedicel (Figure 4h) and such a kink was also seen in the secondary inflorescences (Figure 4i). The ectopic organs became increasingly carpeloid- and then staminoid-like in laterdeveloping flowers (Figure 4j–n). Finally, flowers were completely replaced by staminoid bracts, which were produced in spiral phyllotaxy (Figure 4d,e,j). The inflorescence meristem itself was not consumed and stayed indeterminate (Figure 4e). Together, these phenotypes suggest a role of AGL6 both in suppressing outgrowth of the cryptic bract and in organ identity. |
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5楼2013-08-05 11:07:55
bullfrog325
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【答案】应助回帖
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感谢参与，应助指数 +1
137167741: 金币+2, 小木虫交流，共同进步~~ 2013-08-02 08:25:12
感谢参与，应助指数 +1
137167741: 金币+2, 小木虫交流，共同进步~~ 2013-08-02 08:25:12
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说说我的看法, 其实我不太赞同文章的这个结论. VP16融合的结果是让AGL6强力启动它控制的基因(抑制的, 促进的全都超表达). 所以文章描述苞叶长出的情况也有可能是本身被AGL6促进的基因在有VP16的情况下表达过了头造成的. 在这种情况下, 就不能说AGL6抑制苞叶生长了. 但是也不能说文章写错了, 因为原文用的是"suggest", 还是给自己留了后路的...... 真要支持文章结论的话应该再补些实验, 如在长苞叶的突变体里面把AGL6 KO掉, 看看是否苞叶长出会被削弱. |
2楼2013-08-01 23:10:13
3楼2013-08-02 08:11:33
bullfrog325
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4楼2013-08-02 15:51:10
