【答案】应助回帖

1楼2013-03-22 08:42:21

aixia5

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【答案】应助回帖

感谢参与，应助指数 +1

细胞一般用 β-Actin ，GAPDH,

2楼2013-03-22 09:09:49

gyesang

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【答案】应助回帖

感谢参与，应助指数 +1

内参抗体，有抗-GAPDH(36KD)，beta-actin(42KD), beta-tubulin(55KD)

常用核内参抗 lamin A/B1，PCNA，TATA-box bingding protein（TBP）

常用的标签抗体，有抗-c-myc, 6XHis, GFP, Flag, HA

特点，不知道你是想问抗体的特点还是蛋白的特点，你可以把问题问的更清楚些

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3楼2013-03-22 11:19:11

小米一粒m

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引用回帖:

3楼: Originally posted by gyesang at 2013-03-22 11:19:11
内参抗体，有抗-GAPDH(36KD)，beta-actin(42KD), beta-tubulin(55KD)

常用核内参抗 lamin A/B1，PCNA，TATA-box bingding protein（TBP）

常用的标签抗体，有抗-c-myc, 6XHis, GFP, Flag, HA

特点，不知道 ...

比如说细胞总蛋白，细胞核和细胞膜以及线粒体中的内参分别是什么？分子量大小分别是多少?我在做一个汇总表格

4楼2013-03-25 15:17:18

2240423995

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【答案】应助回帖

楼上的不用做了，可以用Abbkine的，他们很详细的。
内参抗体名称内参类型观察分子量品牌货号详情
Beta-Actin mAb (1C7) 细胞总蛋白 42 kD Abbkine A01010 小鼠源单克隆
Beta-Actin mAb (1C7) , HRP 细胞总蛋白 42 kD Abbkine A01015 小鼠源单克隆,HRP偶联
GAPDH mAb (2B5) 细胞总蛋白 36 kD Abbkine A01020 小鼠源单克隆
GAPDH mAb (2B5) , HRP 细胞总蛋白 36 kD Abbkine A01025 小鼠源单克隆,HRP偶联
Beta-Tubuline mAb (3G6) 细胞总蛋白 55 kD Abbkine A01030 小鼠源单克隆
Plant Actin mAb (3T3) 植物细胞总蛋白 42 kD Abbkine A01050 小鼠源单克隆
PCNA mAb (1D7) 细胞核蛋白 28 kD Abbkine A01040 小鼠源单克隆
COX IV mAb (14Y2) 细胞线粒体总蛋白 16 kD Abbkine A01060 小鼠源单克隆
Histone H3 mAb (2D10) 细胞核蛋白 18 kD Abbkine A01070 小鼠源单克隆

这个是所有内参抗体列表你可以去http://amyjet.com/techniques/why-use-internal-control.shtml 查看

5楼2013-10-01 21:05:15

oggyage

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【答案】应助回帖

楼上说的很好，Abbkine的内参抗体很好，生物帮上有个关于他的专题，内容很详细http://www.bio1000.com/zt/product/475336.html

应该是武汉艾美捷在做。

6楼2013-11-01 11:05:44

majimayi

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【答案】应助回帖

Proteintech好抗体西美杰助力好科研
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7楼2015-12-07 09:39:23