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yuechuan86

银虫 (初入文坛)

[求助] NCBI提交序列后是否遇到这个问题

本人提交了一条基因序列到GenBank里,现在今天收到回信说我的序列有问题。但是我的序列是测了几次的啊!麻烦各位看看,给提个意见!
来信如下:
We are currently processing your GenBank record xxxxxxx
and have come across an issue that requires your attention.

Upon doing a BLAST similarity search of your sequence, we noticed
that there may be a reading frame shift in the translation of the
xxxxxx gene around nt 1196, relative to similar proteins. This appears
to results in a protein that is shorter at the carboxy-temrinus.

If the nucleotide sequence is incorrect, send us the corrected
sequence, and we will replace the current sequence in your
submission.

If the sequence is correct, please let us know if you have any
evidence that this frame shifted protein is translated.

我再次BLAST过,结果里也有的序列和我的一样的!!!!
给个建议吧
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yuechuan86

银虫 (初入文坛)

谢谢啦,我BLAST结果后,用其他的同源基因序列BLAST,有的也有这种情况的
3楼2013-03-08 16:20:29
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niil

金虫 (正式写手)

【答案】应助回帖

感谢参与,应助指数 +1
他们的blast分析是和其他物种的同源蛋白做的比较,通过比较能发现序列有移码。这样就造成了它的C端短一截。这个需要你自个的分析去确定这个位置的移码存在的原因。有可能这个基因就是这个样子的。如果是真的有问题的话,可能是测序出现的问题,或者是在序列拼接的过程中出现的问题。可以查一下原始的测序峰图,剔除掉低质量序列之后再拼接。
Stop fighting,let it flow.
2楼2013-03-08 11:49:36
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