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http://pubs.acs.org/doi/abs/10.1021/ac303204q
Approximately 5 mg of MNS or FNS were activated
in 100 mM EDC and 100 mM NHS in l mL of 0.01 M PBS
(pH 6.8) at room temperature with continuous shaking. After
incubating for 30 min, MNS were separated by magnetic force,
and FNS by centrifugation, and washed with 0.01 M PBS (pH
7.2) three times. Then, they were dispersed in 1 mL of 0.01 M
pH 7.2 PBS to react with 2 mg of NH2−PEG−CM for about 4
h at room temperature with continuous shaking. Afterward, the
resultant MNS−PEG−COOH and FNS−PEG−COOH were
washed by 0.01 M PBS (pH 7.2) five times to remove any
unreacted NH2−PEG−CM. Then, MNS−PEG−COOH and
FNS−PEG−COOH were reacted with the antibodies to S.
typhimurium (about 50 ¦Ìg), and the conjugation procedure was
similar to that above except that the NH2−PEG−CM were
replaced by antibodies
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