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a Select at least three substantially different ionization principles that are used to introduce solutes of varying nature (small organics vs. large biomolecules, non- polar vs. polar, etc) into a mass spectrometer and discuss each of these in relation to a) their ability to produce gas phase ions of solutes of different molecular size, b) the level of fragmentation resulting from the processes and c) how the gas phase ions eventually detected by the mass spectrometer can be used to unravel the identity of solutes. (5 p) b Describe, preferably with the aid of figures (if you ¡±borrow¡± these from a book or from the Internet, please do not forget to give a proper reference) the operational principles of most commonly encountered mass analysers (sector instruments, quadrupoles including ion traps, time-of-flight based measurements, and FT-ICR) that are used in MS. Round off by a short discussion of the relative merits of these mass filter principles (here a table might be useful) in terms of mass resolution and MW limit, in relation to the cost and complexity of their operation. |
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