| 查看: 1112 | 回复: 4 | ||
| 当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖 | ||
[求助]
亲~~翻译一下~~谢谢了~~
|
||
|
Efficient Insertion Mutagenesis Strategy for Bacterial Genomes Involving Electroporation of In Vitro-Assembled DNA Transposition Complexes of Bacteriophage Mu An efficient insertion mutagenesis strategy for bacterial genomes based on the phage Mu DNA transposition reaction was developed. Incubation of MuA transposase protein with artificial mini-Mu transposon DNA in the absence of divalent cations in vitro resulted in stable but inactive Mu DNA transposition complexes, or transpososomes. Following delivery into bacterial cells by electroporation, the complexes were activated for DNA transposition chemistry after encountering divalent metal ions within the cells. Mini-Mu transposons were integrated into bacterial chromosomes with efficiencies ranging from 104to 106CFU/g of inputtransposon DNA in the four species tested, i.e., Escherichia coli, Salmonella entericaserovar Typhimurium, Erwinia carotovora , and Yersinia enterocolitica . Efficiency of integration was influenced mostly by the competence status of a given strain or batch of bacteria. An accurate 5-bp target site duplication flanking the transposon, a hallmark of Mu transposition, was generated upon mini-Mu integration into the genome, indicating that a genuine DNA transposition reaction was reproduced within the cells of the bacteria studied. This insertion mutagenesis strategy for microbial genomes may be applicable to a variety of organisms provided that a means to introduce DNA into their cells is available |
回复此楼» 猜你喜欢
304求调剂
已经有6人回复
-
材料工程专硕调剂
已经有6人回复
-
一志愿天大材料与化工（085600）总分338
已经有4人回复
-
085700资源与环境308求调剂
已经有3人回复
-
求材料调剂
已经有8人回复
-
294求调剂材料与化工专硕
已经有5人回复
-
一志愿华中科技大学，080502，354分求调剂
已经有4人回复
-
一志愿吉林大学材料学硕321求调剂
已经有6人回复
-
085410人工智能专硕317求调剂（0854都可以）
已经有3人回复
-
330求调剂
已经有3人回复
» 本主题相关价值贴推荐，对您同样有帮助:
- 帮忙翻译一下，谢谢
已经有3人回复
- 帮忙翻译一下，谢谢
已经有5人回复
- 帮忙翻译一下 专业的 谢谢
已经有2人回复
- 帮忙翻译一下 谢谢
已经有4人回复
- 请大家尽快帮翻译一下，谢谢。
已经有1人回复
- 帮忙译一下，谢谢~~
已经有1人回复
- 帮忙译一下，谢谢~~
已经有3人回复
- 帮忙翻译一下！谢谢
已经有1人回复
- 请帮忙翻译一下，谢谢
已经有4人回复
- 哪位高手能帮忙翻译一下吗？谢谢
已经有2人回复
ailing2023
铁杆木虫 (知名作家)
美食顾问小橙
- 外语EPI: 10
- 应助: 114 (高中生)
- 贵宾: 0.775
- 金币: 7890.1
- 散金: 9713
- 红花: 86
- 沙发: 77
- 帖子: 9636
- 在线: 844小时
- 虫号: 1197205
- 注册: 2011-01-28
- 性别: MM
- 专业: 天然药物化学
4楼2012-07-05 16:47:11
yangbof
木虫 (著名写手)
- 应助: 2 (幼儿园)
- 金币: 17319.1
- 散金: 185
- 红花: 3
- 帖子: 1443
- 在线: 519小时
- 虫号: 1232312
- 注册: 2011-03-14
- 性别: GG
- 专业: 电化学
3楼2012-07-03 11:32:29
5楼2012-07-06 19:59:01
5