| 查看: 885 | 回复: 11 | |||
[交流]
交流
|
请问虫子们：你们有没有做过油脂里的总酚含量测定？我们现在测遇到了一些问题，很无助，周围都没有人在做，不知道该怎么继续了？ |
回复此楼» 猜你喜欢
【实验干货】生物超薄切片+HRTEM：线粒体嵴原子像一步到位
已经有0人回复
-
食品专业论文投稿请教
已经有0人回复
-
食品科学论文润色/翻译怎么收费?
已经有104人回复
-
分享食品专业书籍《Fundamentals of Food Process Engineering》，以学习目的为主。
已经有2人回复
-
Biomass Conversion and Biorefinery
已经有4人回复
» 抢金币啦！回帖就可以得到:
-
澳大利亚科廷大学招收全奖博士（采矿与冶金工程专业）
+1/284
-
87 年东北人定居苏州，诚寻携手余生的你
+1/162
-
寻找那个能让“稳定生活”变得“更有趣”的你
+1/162
-
吉林师范大学宽禁带半导体材料生长与器件实验室招收2026年入学申请考核制博士生4名
+2/84
-
山东理工大学材料学院泰山学者青年专家招收2026年调剂硕士研究生
+1/79
-
中山大学化学工程与技术学院杰青欧阳钢锋/吴鑫课题组博士后招聘启事
+1/45
-
同济大学 环境科学与工程学院 国家级青年人才团队 诚聘脱产博士后
+1/29
-
澳门理工大学人工智能智慧康养2026 年9月入学 博士招生有奖学金
+1/29
-
211大学【2026学博】补招
+1/28
-
中国林科院林业研究所/林木遗传育种全国重点实验室 “推免生”硕士/博士生招生
+1/20
-
26年启明计划
+1/17
-
【少数民族】【生物医药】2026青岛大学招博士生
+1/11
-
北京某研究院结构生物学相关专业申请考核制博士招生（春季）
+1/8
-
邀稿综述，作者排名和工作量分工问题，存在不满和疑问。
+1/8
-
诚邀有志之士加入江苏大学环境学院合成生物学课题组！(长期接收学硕专硕、硕博连读)
+1/8
-
海南大学-国家级人才团队 招2026级全日制硕士研究生
+1/7
-
斯德哥尔摩博士后基金申请 - 基于视频的三维人体运动学提取 / 姿态估计
+1/3
-
致海外生物医药博士｜科研成果转化，诚邀携手合作
+1/3
-
天津大学接收读博申请！！！
+1/3
-
中科院合肥物质研究院招聘水系锌电博士后（长期有效）诚聘海内外优秀博士后
+1/2
2楼2012-04-25 15:21:02
3楼2012-04-25 15:37:24
4楼2012-04-25 15:42:23
5楼2012-04-25 15:49:12
|
是的，福林酚法，我们是测油的总酚含量，先用正己烷和甲醇提取出来，再测，按照文献上的操作，但是吸光度却很低，假若增加样品溶液的量又会产生沉淀。Briefly for the extraction of phenolic compounds, 10 g of oil was mixed with hexane (50 ml). This mixture was placed into a separation funnel and 20 ml of methanol: water (60:40, w:w) was added. The mixture was shaken for 2 min and the separated water phase was removed. This procedure was applied three times and all the water phases were combined and used for TP analysis. For TP analysis, the mixture of the sample solution (50 ll), distilled water (3 ml), 250 ll Folin–Ciocalteu’s reagent solution, and 7% Na2CO3 (750 ll) was vortexed and incubated for 8 min at room temperature. Then 950 ll of distilled water was added. The mixture was allowed to stand for 2 h at room temperature. The absorbance was measured at 765 nm against distilled water as blank |
6楼2012-04-25 17:38:58
7楼2012-04-25 18:15:37
8楼2012-04-25 20:37:07
|
我们是测油的总酚含量，先用正己烷和甲醇提取出来，再测，按照文献上的操作，但是吸光度却很低，才0.08左右，代到标曲里面算到的总酚含量也很低，明显错的。假若增加样品溶液的量又会产生沉淀。方法是：Briefly for the extraction of phenolic compounds, 10 g of oil was mixed with hexane (50 ml). This mixture was placed into a separation funnel and 20 ml of methanol: water (60:40, w:w) was added. The mixture was shaken for 2 min and the separated water phase was removed. This procedure was applied three times and all the water phases were combined and used for TP analysis. For TP analysis, the mixture of the sample solution (50 ll), distilled water (3 ml), 250 ll Folin–Ciocalteu’s reagent solution, and 7% Na2CO3 (750 ll) was vortexed and incubated for 8 min at room temperature. Then 950 ll of distilled water was added. The mixture was allowed to stand for 2 h at room temperature. The absorbance was measured at 765 nm against distilled water as blank |
9楼2012-04-25 22:48:24
10楼2012-04-25 23:26:11
11楼2012-04-26 08:20:54
12楼2012-04-27 22:35:27