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请问虫子们：你们有没有做过油脂里的总酚含量测定？我们现在测遇到了一些问题，很无助，周围都没有人在做，不知道该怎么继续了？ |
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2楼2012-04-25 15:21:02
3楼2012-04-25 15:37:24
4楼2012-04-25 15:42:23
5楼2012-04-25 15:49:12
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是的，福林酚法，我们是测油的总酚含量，先用正己烷和甲醇提取出来，再测，按照文献上的操作，但是吸光度却很低，假若增加样品溶液的量又会产生沉淀。Briefly for the extraction of phenolic compounds, 10 g of oil was mixed with hexane (50 ml). This mixture was placed into a separation funnel and 20 ml of methanol: water (60:40, w:w) was added. The mixture was shaken for 2 min and the separated water phase was removed. This procedure was applied three times and all the water phases were combined and used for TP analysis. For TP analysis, the mixture of the sample solution (50 ll), distilled water (3 ml), 250 ll Folin–Ciocalteu’s reagent solution, and 7% Na2CO3 (750 ll) was vortexed and incubated for 8 min at room temperature. Then 950 ll of distilled water was added. The mixture was allowed to stand for 2 h at room temperature. The absorbance was measured at 765 nm against distilled water as blank |
6楼2012-04-25 17:38:58
7楼2012-04-25 18:15:37
8楼2012-04-25 20:37:07
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我们是测油的总酚含量，先用正己烷和甲醇提取出来，再测，按照文献上的操作，但是吸光度却很低，才0.08左右，代到标曲里面算到的总酚含量也很低，明显错的。假若增加样品溶液的量又会产生沉淀。方法是：Briefly for the extraction of phenolic compounds, 10 g of oil was mixed with hexane (50 ml). This mixture was placed into a separation funnel and 20 ml of methanol: water (60:40, w:w) was added. The mixture was shaken for 2 min and the separated water phase was removed. This procedure was applied three times and all the water phases were combined and used for TP analysis. For TP analysis, the mixture of the sample solution (50 ll), distilled water (3 ml), 250 ll Folin–Ciocalteu’s reagent solution, and 7% Na2CO3 (750 ll) was vortexed and incubated for 8 min at room temperature. Then 950 ll of distilled water was added. The mixture was allowed to stand for 2 h at room temperature. The absorbance was measured at 765 nm against distilled water as blank |
9楼2012-04-25 22:48:24
10楼2012-04-25 23:26:11
11楼2012-04-26 08:20:54
12楼2012-04-27 22:35:27