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请问虫子们：你们有没有做过油脂里的总酚含量测定？我们现在测遇到了一些问题，很无助，周围都没有人在做，不知道该怎么继续了？

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1楼 2012-04-25 15:10:53

已阅回复此楼关注TA 给TA发消息送TA红花 TA的回帖

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引用回帖:

8楼: Originally posted by myselfsky8159 at 2012-04-25 20:37:07:
遇到什么问题了？

我们是测油的总酚含量，先用正己烷和甲醇提取出来，再测，按照文献上的操作，但是吸光度却很低，才0.08左右，代到标曲里面算到的总酚含量也很低，明显错的。假若增加样品溶液的量又会产生沉淀。方法是：Briefly for the extraction of phenolic compounds,
10 g of oil was mixed with hexane (50 ml). This mixture
was placed into a separation funnel and 20 ml of methanol:
water (60:40, w:w) was added. The mixture was
shaken for 2 min and the separated water phase was
removed. This procedure was applied three times and all
the water phases were combined and used for TP analysis.
For TP analysis, the mixture of the sample solution
(50 ll), distilled water (3 ml), 250 ll Folin–Ciocalteu’s
reagent solution, and 7% Na2CO3 (750 ll) was vortexed
and incubated for 8 min at room temperature. Then
950 ll of distilled water was added. The mixture was
allowed to stand for 2 h at room temperature. The
absorbance was measured at 765 nm against distilled
water as blank