| 查看: 406 | 回复: 0 | |||
[求助]
求教！做western blot实验的抗体如何选择？
|
|
我想做药物作用于细胞后，细胞信号传导途径的Wnt途径中的靶基因cyclin D1的表达情况，cyclin D1是作用于G1期的重要的细胞周期蛋白，但是在抗体选择方面， 不知道cyclin D1（L283）PAb 与cyclin D1（D86）这两种抗体有何不同？是否都可以用于Wnt途径中的靶基因cyclin D1的表达情况？L和D指的是什么？ 这是两种抗体的简要说明： cyclin D1（L283）PAb The proliferation of eukaryotic cells is controlled at specific points in the cell cycle, particularly at the G1 to S and the G2 to M transitions. It is well established that the Cdc2 p34-cyclin B protein kinase plays a critical role in the G2 to M transition while cyclin A associates with Cdk2 p33 and functions in S phase. Considerable effort directed towards the identification of G1 cyclins has led to the isolation of cyclin D, cyclin C and cyclin E. Of these, cyclin D corresponds to a putative human oncogene, designated PRAD1, which maps at the site of the Bcl1 rearrangement in certain lymphomas and leukemias. Two additional human type D cyclins, as well as their mouse homologs, have been identified. Evidence has established that members of the cyclin D family function to regulate phosphorylation of the retinoblastoma gene product, thereby activating E2F transcription factors. cyclin D1（D86） During each cell cycle cyclins undergo periodic accumulation and destruction. As key regulators of the cell cycle the cyclins control important transitions by acting as regulatory subunits of the Cdks. Early in the G1 phase of the cell cycle, cyclin D1 induction is followed by cyclin E induction. This sequential progression is marked early on in G1 by the activation of Cdk4 and in mid to late G1 by the activation of Cdk2 and the hyperphosphorylation of pRB. The final transition into S phase is thought to be dependent on the increased expression and association of cyclin E and Cdk2. |
回复此楼» 收录本帖的淘帖专辑推荐
 生物科学与技术 |
» 猜你喜欢
材料调剂
已经有7人回复
-
化学工程085602 305分求调剂
已经有10人回复
-
289求调剂
已经有15人回复
-
291 求调剂
已经有7人回复
-
274求调剂
已经有14人回复
-
北京林业大学硕导招生广告
已经有3人回复
-
309求调剂
已经有5人回复
-
292求调剂
已经有8人回复
-
求调剂
已经有4人回复
-
一志愿 西北大学 总分282 英语一62 求调剂
已经有3人回复
找到一些相关的精华帖子，希望有用哦~
Western Blot实验的蛋白条带都连在一起
已经有37人回复
- western blot 求助，请高手指点一下
已经有8人回复
- 关于Western blot的抗体孵育阶段
已经有7人回复
- 【求助/交流】western blot
已经有35人回复
- 【求助/交流】western blot的内参总是不齐怎么办
已经有19人回复
- 【求助】虚心向大家求教western blot
已经有4人回复
- 原创首发：Tips for Western Blot-一些小技巧而已
已经有13人回复
点击这里搜索更多相关资源科研从小木虫开始，人人为我，我为人人
