²é¿´: 6078  |  »Ø¸´: 11

δĩ

гæ (³õÈëÎÄ̳)

[ÇóÖú] Çë½ÌsiRNAתȾµÄ¼¸¸öÎÊÌâ ÒÑÓÐ3È˲ÎÓë

¸÷λսÓѺÃ~~~~~ÎÒÊÇÒ»ÃûÑо¿ÉúÐÂÉú£¬±¯´ßµÄÒª×ösiRNA£¬µ«ÊÇÎÒÃÇʵÑéÊÒÓÖûÓÐÕâ·½Ãæ¾­ÑéºÍ¼¼Êõ£¬ÓÚÊÇÖ»ÄÜ×Ô¼ºÃþË÷ÁË¡£¡£ÓÐЩ²ËÄñ¼¶µÄÎÊÌ⣬»¹Çë¸÷λר¼Ò¸ßÊÖ²»Áߴͽ̣¡£¡£¡

     
       ¢ÙÔÚ×ösiRNAµçÓ¾ºÍתȾµÈµÈʵÑéʱ£¬ÑªÇ壬ÅàÑøÒº£¬ÒÈøµÈÅàÑøÎïÖÊÈçºÎÈ¥³ýRNase£¿ »¹ÊÇпª·âµÄÓ¦¸ÃûÓÐRNaseÄØ£¬µ«ÊÇÓÃÁ˼¸´ÎÖ®ºóÄØ£¿
       ¢ÚsiRNAºÜÈÝÒ×½µ½â£¬ÄÇô¿ÉÒÔ¶Ôº¬siRNAµÄ¾ÛºÏÎïÄÉÃ×Á£×öÖîÈçÁ£¾¶¡¢Í¸Éäµç¾µ¡¢×ÏÍâµÈµÈʵÑéÂð£¨Ä¿µÄÊÇ¿¼²ìsiRNA¸úÎÒÃÇʵÑéÓþۺÏÎ︴ºÏ³ÉµÄÄÉÃ×Á£µÄÐÔÖÊ£©£¿
       ¢ÛsiRNA ¸úDNAµÄÐÔÖʲî±ðÓжà´ó£¿DNAµÄתȾ¿É·ñÄ£ÄâsiRNA?×öתȾʵÑé¿ÉÒÔÏÈÓÃeGFP,PGL-3Ö®ÀàµÄDNA±¨¸æ»ùÒòÀ´¼ì²âÎҵľۺÏÎïµÄתȾЧÂÊÂð£¿
       ¢Ü¹ØÓÚתȾŨ¶È¡£Ô°×ÓÀïµÄר¼ÒÃÇ˵siRNAµÄ×÷ÓÃŨ¶ÈÊÇ10~100nM¡£µ«ÊÇÎÒÃÇ´Ó¹«Ë¾ÂòÀ´µÄ×ܹ²²Å5nmol£¬¾ÍÒª½üÁ½Ç§¿éÁË£¬ÕâôÓÃÔõô³ÔµÃÏû°¡¡£¡£¡£ÇóÖúŨ¶ÈÓÃpmol¼¶µÄ¿ÉÒÔÂð£¿
»Ø¸´´ËÂ¥

» ²ÂÄãϲ»¶

» ±¾Ö÷ÌâÏà¹ØÉ̼ÒÍÆ¼ö: (ÎÒÒ²ÒªÔÚÕâÀïÍÆ¹ã)

» ±¾Ö÷ÌâÏà¹Ø¼ÛÖµÌùÍÆ¼ö£¬¶ÔÄúͬÑùÓаïÖú:

ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

δĩ

гæ (³õÈëÎÄ̳)

×Ô¼º¶¥Ò»ÏÂ~~
2Â¥2011-11-10 15:58:43
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

2011¼ÓÓÍ

Òø³æ (ÕýʽдÊÖ)

ÎÒÒ²ÏëÉè¼ÆsiRNA£¬ÇëÎÊÄãÊÇÔõôÉè¼Æ£¬À´È·¶¨ÏëÒªµÄsiRNAƬ¶Î£¿Ð»Ð»»Ø´ð£¬ÆÚ´ý»Ø¸´¡£
ÿÌì¶¼ÊÇпªÊ¼£¬2011£¬ÒªºÃºÃŬÁ¦£¡
3Â¥2011-11-11 14:32:22
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

δĩ

гæ (³õÈëÎÄ̳)

ÒýÓûØÌû:
3Â¥: Originally posted by 2011¼ÓÓÍ at 2011-11-11 14:32:22:
ÎÒÒ²ÏëÉè¼ÆsiRNA£¬ÇëÎÊÄãÊÇÔõôÉè¼Æ£¬À´È·¶¨ÏëÒªµÄsiRNAƬ¶Î£¿Ð»Ð»»Ø´ð£¬ÆÚ´ý»Ø¸´¡£

Õâ¸ö²»ÊÇÎÒÉè¼ÆµÄ£¬ºÇºÇ£¬²»ºÃÒâ˼ÁË~~տɣÐÄ£¬Ä¾ÓÐÈ˻ظ´¡£¡£¡£
4Â¥2011-11-14 13:41:04
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

2011¼ÓÓÍ

Òø³æ (ÕýʽдÊÖ)

ÒýÓûØÌû:
4¥: Originally posted by δĩ at 2011-11-14 13:41:04:
Õâ¸ö²»ÊÇÎÒÉè¼ÆµÄ£¬ºÇºÇ£¬²»ºÃÒâ˼ÁË~~տɣÐÄ£¬Ä¾ÓÐÈ˻ظ´¡£¡£¡£

ÄãÃÇʵÑéÊÒÂòµÄÊÇÏֳɵÄÂð£¿ÕâÒ²ºÜºÃ°¡£¬Ê¡È¥ºÜ¶àÂé·³
ÿÌì¶¼ÊÇпªÊ¼£¬2011£¬ÒªºÃºÃŬÁ¦£¡
5Â¥2011-11-14 20:01:22
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

δĩ

гæ (³õÈëÎÄ̳)

ÒýÓûØÌû:
5Â¥: Originally posted by 2011¼ÓÓÍ at 2011-11-14 20:01:22:
ÄãÃÇʵÑéÊÒÂòµÄÊÇÏֳɵÄÂð£¿ÕâÒ²ºÜºÃ°¡£¬Ê¡È¥ºÜ¶àÂé·³

ÊǵÄѽ˵~~~
6Â¥2011-11-21 10:52:14
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

704361326

½ð³æ (ÕýʽдÊÖ)

¶÷¶÷

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

ÎÒÒ²¸Õ×ö²»¾Ã£¬Ï໥ÌÖÂÛ
1. Õâ¸ö¿ÉÒÔ¿¼ÂÇ£¬µ«Ã»°ì·¨¡£Ç¹¼â¡¢EP¹ÜÀàÖÆÆ·ÒªÓýø¿ÚµÄ¡£Äã¿ÉÒÔ¿´½ø¿ÚepµÄ°ü×°ºÐ£¬ÊÇÈ¥RNaseµÄŶ£¡
2.ÎÒÒ²»á´ó²»ÁË
3. ÎÒ¾õµÃ¿ÉÒÔ£¬ÎÒÒ²Õý×¼±¸ÕâÑù³¢ÊÔ¡£Ô­Òò£ºÄ¿µÄRNAºÜ¹ó£¬ÎÒÓÐÆäËûµÄ£¬¾ÍÏȳ¢ÊÔ×öתȾ£¬ËäÈ»Óвî±ð£¬µ«´óÖÂÏàͨ°¡¡£

ÒÔÉϽ¨Ò飬½ö×÷²Î¿¼¡£
ÎÒÒ²ÔÚ×ösiRNA,ÄãºÃÏñÊÇÒ©¼Á·½ÏòµÄ¡£¶à¶àÌÖÂÛŶ£¡
̤̤ʵʵµØ×ߺÃ×Ô¼ºµÄ·
7Â¥2015-05-26 17:12:19
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

fenggeshj

гæ (СÓÐÃûÆø)

ÒýÓûØÌû:
7Â¥: Originally posted by 704361326 at 2015-05-26 17:12:19
ÎÒÒ²¸Õ×ö²»¾Ã£¬Ï໥ÌÖÂÛ
1. Õâ¸ö¿ÉÒÔ¿¼ÂÇ£¬µ«Ã»°ì·¨¡£Ç¹¼â¡¢EP¹ÜÀàÖÆÆ·ÒªÓýø¿ÚµÄ¡£Äã¿ÉÒÔ¿´½ø¿ÚepµÄ°ü×°ºÐ£¬ÊÇÈ¥RNaseµÄŶ£¡
2.ÎÒÒ²»á´ó²»ÁË
3. ÎÒ¾õµÃ¿ÉÒÔ£¬ÎÒÒ²Õý×¼±¸ÕâÑù³¢ÊÔ¡£Ô­Òò£ºÄ¿µÄRNAºÜ¹ó£¬ÎÒÓÐÆäËûµÄ ...

ÄãºÃ,ÇëÎÊÄãÓÐ×ö¹ýSiRNAµÄϸ°ûÔöֳʵÑéÂð?ÎÒ¸Õ¿ªÊ¼×öSiRNA,²»ÖªµÀ¸ÃÓöàÉÙŨ¶È.Ï£ÍûµÃµ½Ö¸µ¼.лл¡«
8Â¥2015-07-23 09:15:22
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

zbxky

Ìú³æ (СÓÐÃûÆø)

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

siRNAתȾ³£¼ûÎÊÌâ¼°½â¾ö·½°¸

1.        RNAÓëתȾÊÔ¼Á±ÈÀý²»¼Ñ
ÓÉÓÚRNAÐòÁвîÒì¡¢ºÏ³ÉÌõ¼þ²»Í¬ÒÔ¼°ÊÇ·ñ´øÓÐÓ«¹âµÈ±ê¼Ç£¬¾ö¶¨ÁËRNAºÍתȾÊÔ¼ÁÔÚ²»Í¬Çé¿öÏ»áÓв»Í¬µÄ×î¼ÑÌõ¼þ£¬½¨ÒéÏȽøÐÐԤʵÑéÓÅ»¯¡£
RNAŨ¶ÈºÍתȾÊÔ¼ÁÁ¿µÄÓÅ»¯(24well)

1         2         3         4
RNA¹¤×÷Ũ¶È         25nM         50nM         100nM         150nM
ÿ¿×RNAµÄÁ¿(pmol)         0.17¦Ìg
(12.5pmol)         0.33¦Ìg
(25pmol)         0.67¦Ìg
(50pmol)         1¦Ìg
(75pmol)
ÿ¿×תȾÊÔ¼ÁÁ¿         0.25¦Ìl         0.5¦Ìl         1¦Ìl         1.5¦Ìl

2.        ϸ°ûÃܶȲ»¼Ñ
µ÷Õûϸ°ûÃܶȵ½×ªÈ¾Ê±»ãºÏ¶ÈΪ20-40%¡£³É¹¦×ªÈ¾siRNAµÄϸ°û»á²úÉúÄ¿±ê»ùÒò±í´ïϵ÷£¬µ«Î´³É¹¦×ªÈ¾µÄϸ°ûÈ´²»ÊÜÓ°Ï죬ÕâʱתȾЧÂʺÍ×ܵÄϸ°ûÊýÁ¿¾ÍºÜÖØÒª£¬Ò»°ãϸ°ûÊýÁ¿½ÏÉÙʱתȾЧÂʸߡ£ÓÉÓÚsiRNA³ÁĬʱЧÐÔµÄÓ°Ï죬תȾºó48Сʱ²ÅÄܽøÐнøÐÐqRT-PCR¼ì²â£¬×ªÈ¾ºó48-72Сʱ²ÅÄܽøÐе°°×¼ì²â¡£Èç¹ûתȾʱÆÌ°åÃܶȽϸߣ¬Ï¸°ûÒ»·½Ãæ×ªÈ¾Ð§¹û²»ÀíÏ룬ֱ½ÓÓ°Ïì³ÁĬЧ¹ûºÍÊý¾Ý¿É¿¿ÐÔ£¬ÁíÒ»·½Ã棬48СʱÉõÖÁ¸ü³¤Ê±¼äºó£¬³ÁĬ¼ì²â×î¼Ñµãʱ£¬¹ýÓÚÃܼ¯µÄϸ°û½«Ó°Ïìϸ°û״̬£¬´Ó¶øÓ°ÏìʵÑé½á¹û¡£
3.        RNAЧÂʲ»¸ß
Ñ¡Ôñ×îÓÅRNA£¬²¢²ÉÓÃÒÑÖª¸ßЧµÄRNA×ö¶ÔÕÕ¡£siRNAµÄºÏ³ÉÐèҪעÒâµÄÊÇÒ»¶¨ÒªÑ¡Óøߴ¿¶ÈµÄsiRNA£¬siRNAµÄ´¿¶ÈÖ±½Ó¹ØÏµµ½×ªÈ¾Ð§ÂʺͳÁĬЧÂÊ¡£siRNAµÄ¹¤×÷Ũ¶ÈºÍsiRNAµÄÉè¼Æ¡¢Ï¸°ûÖÖÀࡢĿ±ê»ùÒòÓйء£½¨ÒéÒ»¶¨½øÐÐÏà¹ØµÄԤʵÑéÓÅ»¯¸÷Ìõ¼þ¡£
4.        RNAøÎÛȾ
½¨ÒéÎÞRNAøºÍÄÚ¶¾ËصÄÎüÍ·¡¢ÀëÐĹܺÍÈÜÒºµÈ²ÄÁϺÍʵÑéÆ÷¾ß¡£
5.        תȾºóÅàÑøÊ±¼ä²»¹»
ÔÚmRNAˮƽ¿ÉÒÔµ½48СʱÒÔºóÑéÖ¤½á¹û£¬ÔÚµ°°×ˮƽ¿ÉÒÔµ½72СʱºóÑéÖ¤½á¹û¡£siRNAµÄ³ÁĬЧ¹ûÊÇÓÐʱЧÐԵģ¬ÔÚ×î¼ÑµÄʱ¼äµã¹Û²ì£¬»áµÃµ½¸ü¼ÓÃ÷ÏԵijÁĬЧ¹û£¬Õâ¸öʱ¼äµãµÄÈ·¶¨²»ÄÜÒÔͬһÖêϸ°ûתȾDNAµÄ¾­ÑéÈ·¶¨£¬ÒòΪһ¸öÊÇÏûºÄmRNA£¬Ò»¸öÊDzúÉúmRNA¡£¶ÔתȾsiRNA£¬ÔÚºËËáˮƽ£¬×ªÈ¾ºó8-48Сʱ£¬¼´¿É¹Û²ìµ½mRNAˮƽµÄÖð²½Ï½µ£¬Ò»°ãÀ´Ëµ£¬×ªÈ¾ºó48Сʱ½øÐÐqRT-PCR¼ì²âͨ³£»áµÃµ½Æ¯ÁÁµÄ½á¹û¡£
6.        ÅàÑø»ù¶¾ÐÔ
²ÉÓú¬10-20%ѪÇåµÄÈ«ÅàÑø»ù¡£Ê¹ÓÃÖ¬ÖÊÌåµÈתȾÊÔ¼Áʱ£¬ÓÉÓÚº¬ÑªÇåתȾ»á½«ÑªÇåÖеĵ°°×´øÈëϸ°û£¬Òý·¢Ï¸°û¶¾ÐÔ£¬µ¼ÖÂתȾЧÂʽµµÍ£¬¹Ê²»ÄÜÓÐѪÇåתȾ¡£EntransterTMֻŨËõ°ü¹üºËËá²»»á½«ÑªÇå´øÈëϸ°û£¬ËùÒÔ¿ÉÒÔÓÐѪÇåתȾ£¬Í¬Ê±ÓÉÓÚѪÇåµÄ´æÔÚ£¬ÓÐÀûÓÚϸ°ûµÄ״̬ά»¤£¬¹ÊÄÜÌá¸ßתȾЧÂÊ¡£
7.        ϸ°ûÎÛȾ
½¨Òé³¹µ×Çå½àËùÓÐϸ°ûÅàÑøÏà¹ØÓÃÆ·

Ô­ÎÄÀ´Ô´£ºhttp://www.engreen.cn/
9Â¥2015-08-26 10:31:55
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

zbxky

Ìú³æ (СÓÐÃûÆø)

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

¿ÉÒԵ绰×ÉѯӢ¸ñ¶÷010-68489824
10Â¥2015-09-23 09:36:46
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
Ïà¹Ø°æ¿éÌø×ª ÎÒÒª¶©ÔÄÂ¥Ö÷ δĩ µÄÖ÷Ìâ¸üÐÂ
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼ÑÐ] 070303 ×Ü·Ö349Çóµ÷¼Á +3 LJY9966 2026-03-15 5/250 2026-03-16 14:24 by xwxstudy
[¿¼ÑÐ] 0703 ÎïÀí»¯Ñ§µ÷¼Á +3 ÎÒ¿ÉÒÔÉϰ¶µÄ¶Ô 2026-03-13 5/250 2026-03-16 10:50 by ÎÒ¿ÉÒÔÉϰ¶µÄ¶ÔÂ
[¿¼ÑÐ] ±¾ÈË¿¼085602 »¯Ñ§¹¤³Ì ר˶ +9 ²»ÖªµÀ½Ðʲô£¡ 2026-03-15 10/500 2026-03-16 10:04 by houyaoxu
[¿¼ÑÐ] 0856Çóµ÷¼Á +3 ÁõÃÎ΢ 2026-03-15 3/150 2026-03-16 10:00 by houyaoxu
[¿¼ÑÐ] ÖпÆÔº²ÄÁÏ273Çóµ÷¼Á +3 yzydy 2026-03-15 3/150 2026-03-15 21:15 by ms629
[¿¼ÑÐ] 288Çóµ÷¼Á +4 Ææµã0314 2026-03-14 4/200 2026-03-14 23:04 by JourneyLucky
[¿¼ÑÐ] 080500£¬²ÄÁÏѧ˶302·ÖÇóµ÷¼ÁѧУ +4 ³õʶ¿ÉÀÖ 2026-03-14 5/250 2026-03-14 21:08 by peike
[¿¼ÑÐ] 297Ò»Ö¾Ô¸ÉϽ»085600Çóµ÷¼Á +5 Ö¸¼â°ËǧÀï 2026-03-14 5/250 2026-03-14 17:26 by a²»Ò×
[»ù½ðÉêÇë] ÃæÉϺÍÇà»ùÒ»ÑùÏÞ30Ò³²»ºÏÀí +5 wowsunflower 2026-03-10 7/350 2026-03-14 17:21 by kingkocxr
[¿¼ÑÐ] 267Ò»Ö¾Ô¸ÄϾ©¹¤Òµ´óѧ0817»¯¹¤Çóµ÷¼Á +5 SUICHILD 2026-03-12 5/250 2026-03-14 14:53 by jean5056
[¿¼ÑÐ] Ò»Ö¾Ô¸Ö£´ó070303£¬338·Ö£¬Çóµ÷¼Á +4 dadawaf 2026-03-10 5/250 2026-03-14 01:20 by lsw010101
[¿¼ÑÐ] ²ÄÁϹ¤³Ìר˶£¬Ò»Ö¾Ô¸Öйú¿óÒµ´óѧ£¬×Ü·Ö314£¬Çóµ÷¼Á +5 ÎÞи¿É»÷µÄ¾ÞÈË 2026-03-10 5/250 2026-03-14 00:37 by JourneyLucky
[¿¼ÑÐ] 285 Çóµ÷¼Á ×ÊÔ´Óë»·¾³ Ò»Ö¾Ô¸±±¾©»¯¹¤´óѧ +3 δÃû¿¼Éú 2026-03-10 3/150 2026-03-13 23:04 by JourneyLucky
[¿¼ÑÐ] 337Ò»Ö¾Ô¸»ªÄÏÀí¹¤0805²ÄÁÏÇóµ÷¼Á +7 mysdl 2026-03-11 9/450 2026-03-13 22:43 by JourneyLucky
[¿¼ÑÐ] ²ÄÁϹ¤³Ìµ÷¼Á +9 ßäßä¿Õ¿Õ 2026-03-12 9/450 2026-03-13 22:05 by ÐÇ¿ÕÐÇÔÂ
[¿¼ÑÐ] ¿¼Ñе÷¼Á +4 ·Ò´ï46 2026-03-12 4/200 2026-03-13 16:04 by ruiyingmiao
[¿¼ÑÐ] 0856»¯Ñ§¹¤³Ì280·ÖÇóµ÷¼Á +4 shenzxsn 2026-03-11 4/200 2026-03-13 11:55 by ymwdoctor
[¿¼ÑÐ] ¹¤¿Æ0856ר˶»¯Ñ§¹¤³Ì269Äܵ÷¼ÁÂð +10 ÎÒÏë¶ÁÑÐ11 2026-03-10 10/500 2026-03-13 10:14 by Yuyi.
[¿¼ÑÐ] Ò»Ö¾Ô¸£ºÎ人Àí¹¤£¬²ÄÁϹ¤³Ì£¬Ó¢¶þÊý¶þ ×Ü·Ö314 +3 2202020125 2026-03-10 4/200 2026-03-10 13:54 by xiongyaxuan
[¿¼ÑÐ] Êý¶þÓ¢¶þ309·ÖÇëÇóµ÷¼Á +3 dtdxzxx 2026-03-09 4/200 2026-03-09 19:56 by yuningshan
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û