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sunnytrz

木虫 (著名写手)


[交流] 【求助/交流】5金币求助FITC标记蛋白质的具体步骤

FITC标记蛋白质的具体过程,要求有具体的步骤和注意事项,不是试剂盒中的protocol,就是使用实验室常规的操作方法,将已有的FITC标记到已有的蛋白上面,O(∩_∩)O谢谢!

[ Last edited by sunnytrz on 2011-1-17 at 22:32 ]
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xhjggl

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★ ★
小木虫(金币+0.5):给个红包,谢谢回帖交流
rainwander(金币+1):对生工的产品很熟悉啊???~~~鼓励积极参与交流~~~ 2011-01-14 14:16:14
sunnytrz(金币+1): 2011-01-23 20:18:11
买个试剂盒就可以了,上海生工有代理的进口的产品。
HOOK™ Dye Labeling Kit (FITC)
蛋白FITC荧光素标记试剂盒       
Description:
FITC (fluorescein isothiocyanate) is a commonly used fluorescent label for proteins, as it contains the groups required for conjugating to amino, sulfhydryl, imidazoyl, tyrosyl or carbonyl groups of proteins. FITC has a molecular weight of 389, and excitation and emission wavelengths of 494nm and 520nm, respectively, therefore emitting green visible light.This kit utilizes Tube-O-Reactor™, which allows for the reactions to be performed in a single tube, with no loss of essential reagents and minimum hands-on time and effort.
Features:
Dye preweighed and supplied in single use  vials (Also available separately);Suitable for most proteins;Utilizes single Tube Reactor for minimum hands on time.application for Labeling of a green fluorescent dye to proteins and peptides. And Suitable for antibody labeling. Store: -15~-20℃
编号        包装        价格        产地
786-059           5 Reactions        2,709        GBios

[ Last edited by xhjggl on 2011-1-14 at 20:10 ]
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