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        Synthesis of dDNA with Oligo DNA
        V(H2O)= OD(number)*33*20000/Oligo molecular weight (unit: ul)
Oligo DNA-A: 100uM  VH2O=nmolx10=91ul
Oligo DNA-B: 100uM  VH2O=91ul
        Annealing reaction system preparation
Nuclear free water: 40ul
Annealing buffer for DNA Oligo(5x): 20ul
Oligo DNA-A(100uM): 20ul
Oligo DNA-B(100uM): 20ul
Total volume: 100ul
dDNA concentration: 20uM/10uM
        Annealing reaction procedure using PCR
Procedure        Temperature(¡æ)        Time(min)        specification
1        95        2        Oligo denatured
2        0.1¡æ/8s-25¡æ or 1¡æ/90s-25¡æ        Approximate 90        Annealing
3        4        long time        Temperature storage
3Â¥2010-12-09 20:52:18
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4Â¥: Originally posted by fangxingbaixue at 2010-12-09 20:55:45
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