2- Page 12, lines 226 to 228:
"The carotenoids and gum produced by the wild-type strain were closely bound together, which led to difficulties in recovery, requiring large amounts of alcohol."
In fact, the pigment produced by the wild-type strain binds to the gum and confers a yellow color to the product. This implies of course the needs to purify properly using additional steps, such as extra amounts of ethanol. In the opinion of this referee, the large amount of alcohol required is mainly associated with the dilution of the fermentation media rather than with the presence of the yellow pigment.
Õâ¸öÒâ˼ÊÇ˵ÎÒÒýÓÃÎÄÏײ»µ±Â𣿿ÉÊÇÕâ¾ä»°ÎÒûÒýÓòο¼ÎÄÏ×°¡£¬ÊÇÎÒ×Ô¼ºÐ´µÄ£¬ÄѵÀÉó¸åÈËÈÏΪÕâ¾ä»°ºÍÏÂÒ»¾ä»°¶¼ÊÇÒýÓõģ¿¿öÇÒÏÂÒ»¾ä»°ÒýÓõIJο¼ÎÄÏ×Ò²²»ÊÇ˵ÓÃÀ´dilution of the fermentation mediaµÄ¡£ÊDz»ÊÇÒªÕÒµ½²Î¿¼ÎÄÏ×À´Ö§³ÖÕâ¸ö˵·¨ÄØ£¿¿ÉÃ²ËÆÒ²ÕÒ²»µ½ÎÄÏ×À´ËµÃ÷Õâ¸öÎÊÌ⡣ʵÔڸ㲻Ã÷°×£¬¿ÒÇë´ó¼Ò°ïæ·ÖÎöһϡ£Ð»Ð» |