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5Â¥2010-10-07 22:07:22
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BW25113 is a derivative of the F-, ¦Ë-, E. coli K-12 strain BD792 (CGSC6159)[1], which is a derivative of E. coli K-12 W1485. According to the CGSC, BD792 carries rpoS396(Am), but this mutation is not listed in the published genotype for BW25113, as according to Baba 2006 [2], a pseudoreversion rpoS (Q33) allele was transferred from MG1655 into a predecessor of BW25113. BW25113 was used to make the Keio Collection of single-gene knockouts. [2] [1] Available from the Coli Genetics Stock Center: CGSC#7636 The genotype for BW25113 in Datsenko et al. [1] said the strain is lacIQ, but it was later shown to be lacI+ Baba et al. (Supplementary Table 1)[2]. According to the CGSC, lacZ4787(del) has 4 tandem copies of the rrnB transcriptional terminator inserted by gene replacement into the region extending from near the SacII site near the N-terminus of lacZ through the promoter. References See Help:References for how to manage references in EcoliWiki. ¡ü 1.0 1.1 1.2 1.3 Datsenko, KA & Wanner, BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97 6640-5 PubMed EcoliWiki page ¡ü 2.0 2.1 2.2 2.3 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed EcoliWiki page ÏêϸÐÅÏ¢£ºhttp://ecoliwiki.net/colipedia/index.php/BW25113 PS£º¸Ã¾úÖêÊÇÈÕ±¾ÇìÓ¦ÒåÛÓ´óѧ£¨Keio collection)³£ÓÃÓڴ󳦸˾ú»ùÒòÇóýµÄÒ°ÉúÐ;úÖ꣨WT£©¡£ |
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