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Major Revision (28-Apr-2015)£º

Associate Editor Comments to Author:

Associate Editor
Comments to the Author:
(There are no comments.)



Reviewer(s)' Comments to Author:

Reviewer: 1

Comments to the Author
The manuscript by Zhu et al. evaluated attenuated Salmonella vaccine strain harboring htrA and yncD mutations. The manuscript is understandable but there are several critical points in the manuscript.
(1) Authors should describe the reason why authors chose htrA and yncD double mutant strain for Salmonella enterica serovar Parathyphi A vaccine strain more.
(2) Table 2 is the important result. Authors should show the result more adequately. Describe how many times the experiments were repeated and the mean ¡À SD.
(3) How can LD50 be estimated by ¡°logistic regression analysis¡± (page 6, lines 9, 10)?
(4) Many grammatical and careless mistakes are found through the manuscript. I show some examples below.
Page 2, line 9 and others. ¡°the wild-type¡± is not accurate. ¡°the wild-type strain¡± is better.
Page 2, lines 10, 11. Add ¡°a¡± before ¡°human enteric fever vaccine¡± and ¡°vaccine delivery vector¡±.
Page 4, line 14. ¡°Kanamycin¡± reads ¡°kanamycin¡±.
Page 7, line 4. Remove ¡°assay¡± after ¡°ELISA¡±.
Page 8, line 3. ¡°the yncD mutant and the wild-type did¡± should be ¡°the yncD mutant strain and the wild-type strain¡±.
Page 8, line 9. Add ¡±strains¡± before ¡°(Fig. 2b)¡±.
Page 8, line 16. Add ¡°,¡± before ¡°respectively¡±.
Page 8, line 17. ¡°the wild-type is¡± should be ¡°the wild-type strain¡±.
Page 9, line 1. ¡°liter¡± reads ¡°titer¡±.
Page 9, line 3. Remove ¡°assay¡± after ¡°ELISA¡±.
Page 9, line 4. Remove ¡°did¡± after ¡°PBS control¡±.
Page 9, line 9. ¡°mucin mouse model¡± is strange expression. Just use ¡°mouse model¡±.
Page 10, line 5. The sentence, ¡°the mutant strains either lose¡­reduced.¡± is grammatically wrong.
Page 10, line 13. ¡°and was attenuated¡± is grammatically wrong.
Page 10, line 2 from the bottom. ¡°Up to date¡± is strange. ¡°should be to have¡± should be ¡°should have¡±.
Page 11, line 3. Add a space between ¡°under¡± and ¡°development¡±.
References. Some references use the full journal name and others use the abbreviated form. Follow the author instruction.
Figure legends.
Page 16, line 2. ¡°PCR Identification¡± reads ¡°PCR identification¡±.
Page 16, lines 7, 8. ¡°mutant and the wild-type¡± should be ¡°mutant strain and the wild-type strain¡±.
Page 16, line 8. ¡°mucin mouse model¡± should be ¡°mouse model¡±.
Page 16, line 3 from the bottom. ¡°ELISA assay¡± should be ¡°ELISA¡±.
Table 2 (Page 18). In the title of Table 2, ¡°wild-type¡± should be ¡°wild-type strain¡± and ¡°mucin mouse model¡± should be ¡°mouse model¡±.
Figure 2 (Page 20). The label of y-axis (Lg (CFU/ml)) should be changed. Explanation of ¡°p<0.05¡± is necessary. Which groups were compared?
Figure 3 (Page 21). It is not necessary to show bar graphs to show LD50. A table is better. Describe SD or SEM.



Reviewer: 2

Comments to the Author
The authors present evidence that double deletion of htrA and yncD on genome of Salmonella enterica serovar Paratyphi A resulted in remarkable decreased virulence compared with that of wild type using mucin mice model. They further show that the double deleted mutant is a candidate as an attenuate vaccine of S. Paratyphi A infection.
This is an interesting work of S. Paratyphi A infection and vaccine. This reviewer has a few questions;

1. Usually, S. Pratyphi A could not infect mouse. The authors should explain about the mucin mice model which they assessed S. Paratyphi A virulence. How does S. Paratyphi A kill mice? Does S. Paratyphi A increase in any organs in mice?

2. It is interested that which gene (htrA or yncD) more contribute to the pathogenicity of S. Paratyphi A. The authors should examine the effect of each gene deletion on S. Paratyphi A virulence.

--------------------------------------------------------ÎÒÊÇ»ªÀöÀöµÄ·ÖË®Áë-----------------------------------------------------------

Minor Revision (09-Jun-2015)£º

Associate Editor Comments to Author:

Associate Editor
Comments to the Author:
(There are no comments.)


Reviewer(s)' Comments to Author:

Reviewer: 1

Comments to the Author
The manuscript by Zhu et al. evaluated attenuated Salmonella vaccine strain harboring htrA and yncD mutations. The manuscript was significantly improved after revision.
I just suggest several corrections.
Page 1, line 20. ¡°Lipopolysaccharide¡± reads ¡°lipopolysaccharide¡±.
Page 8, line 164. Remove ¡°Co. Ltd.¡± Add location of the company.
Page 9, line 169. ¡°Independent-samples T test¡± should be ¡°Unpaired t test¡±.
Page 9, line 185. ¡°bacterial sensitivity¡± should be ¡°bacterial vulnerability¡±.
Page 10, line 191. ¡°a 33.3% of mortality rate¡± should be ¡°a mortality rate of 33.3%¡±.
Page 11, line 229. ¡°produced¡± should be ¡°observed¡±.
Page 12, line 244. ¡°stressed¡± reads ¡°stresses¡±.
Page 13, line 267. ¡°date¡± reads ¡°data¡±.
Page 13, line 269. Use ¡°as¡­so far¡± instead of ¡°by now¡±.
Page 20, line 401. ¡°Independent-samples T test¡± should be ¡°Unpaired t test¡±.
Page 21, line 420. ¡°the wild-type strains¡± reads ¡°the wild-type strain¡±.
Page 22, Figure 1. Schemas of Figure 1a and Figure 1b are opposite. DK primers are for yncD gene and HK primers are for htrA gene.
Page 27, Table 1. It is better to use capitals (A, G, C, T) instead of small letters (a, g, c, t) for the oligonucleotide sequences.


Reviewer: 2

Comments to the Author
Dear Authors,

Thank you for the revised manuscript. It is shown clearly for my comments.

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4Â¥: Originally posted by nono2009 at 2015-06-10 19:09:48
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4Â¥: Originally posted by nono2009 at 2015-06-10 19:09:48
Major Revision (28-Apr-2015)£¬ËµÃ÷±à¼­¸øµÄÊÇ´óÐÞ

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Minor Revision (09-Jun-2015)£º

Associate Editor Comments to Author:

Associate Editor
Comments to the Author:
(There are no comments.)


Reviewer(s)' Comments to Author:

Reviewer: 1

Comments to the Author
The manuscript by Zhu et al. evaluated attenuated Salmonella vaccine strain harboring htrA and yncD mutations. The manuscript was significantly improved after revision.
I just suggest several corrections.
Page 1, line 20. ¡°Lipopolysaccharide¡± reads ¡°lipopolysaccharide¡±.
Page 8, line 164. Remove ¡°Co. Ltd.¡± Add location of the company.
Page 9, line 169. ¡°Independent-samples T test¡± should be ¡°Unpaired t test¡±.
Page 9, line 185. ¡°bacterial sensitivity¡± should be ¡°bacterial vulnerability¡±.
Page 10, line 191. ¡°a 33.3% of mortality rate¡± should be ¡°a mortality rate of 33.3%¡±.
Page 11, line 229. ¡°produced¡± should be ¡°observed¡±.
Page 12, line 244. ¡°stressed¡± reads ¡°stresses¡±.
Page 13, line 267. ¡°date¡± reads ¡°data¡±.
Page 13, line 269. Use ¡°as¡­so far¡± instead of ¡°by now¡±.
Page 20, line 401. ¡°Independent-samples T test¡± should be ¡°Unpaired t test¡±.
Page 21, line 420. ¡°the wild-type strains¡± reads ¡°the wild-type strain¡±.
Page 22, Figure 1. Schemas of Figure 1a and Figure 1b are opposite. DK primers are for yncD gene and HK primers are for htrA gene.
Page 27, Table 1. It is better to use capitals (A, G, C, T) instead of small letters (a, g, c, t) for the oligonucleotide sequences.


Reviewer: 2

Comments to the Author
Dear Authors,

Thank you for the revised manuscript. It is shown clearly for my comments.
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