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2Â¥2015-05-25 20:22:15
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genhunter

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ºÎάÄÈ: ½ð±Ò+3, ·­ÒëEPI+1, 3·Ö²»ÄÜÔÙ¶àÁË 2015-05-25 20:48:45
±¾ÊµÑéͨ¹ýTriton X-100£¯Õý¼º´¼£¯»·¼ºÍ飯FITCË®ÈÜÒº×é³ÉµÄ·´Ïà΢ÈéÒºÌåϵ£¬·ÅÖÃ24Сʱºó£¬¾­±ûÍªÆÆÈé¡¢ÀëÐÄ¡¢³¬Éù£¬ÖƱ¸ÁË FITC ²ôÔӵĶþÑõ»¯¹èÓ«¹â¸´ºÏÄÉÃ×Á£×Ó£¬¶Ô¿Ç¾ÛÌÇ¡¢È¾ÁÏÓÃÁ¿½øÐÐÓÅ»¯£¬Í¨¹ý¶ÔÄÉÃ×Á£×ӵıíÕ÷£¬·¢ÏÖÄÉÃ×Á£×Ó°ü¹üµÄȾÁÏÁ¿²»ÊÇÔ½´óÔ½ºÃ£¬¶øÊÇÓÐÒ»¸ö×îÓÅÖµ¡£×îºóÀûÓÃÂÈÀë×Ó¶ÔÓ«¹âÄÉÃ×Á£×ӵĴãÃð×÷Ó㬽øÐÐÒ»¸ö¼òµ¥µÄÓ¦ÓÃ.
In this study we prepared a reverse micelle system composed of TritonX-100/hexanol/cyclohexane/FITC in water (where was chitosan?). After 24hrs, aceton was added to break the emulsion. After centrifuge, ultrasound treatment, FITC incorporated fluorescence SiO2 composite nanopartilces. We did optimization on the amounts of chitosan and the dye and characterized the nanoparticles. We found that there is an optimal amount of the dye that is incorporated, and poorer results were obtained when it exceeded the this amount.  Finally, we tested the quenching effect of chloride ion on the fluorescence nanoparticles and demonstrated it can be used in a simple manner. רҵ°É£¿
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3Â¥2015-05-25 20:42:44
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2Â¥: Originally posted by luo06009228 at 2015-05-25 20:22:15
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4Â¥2015-05-25 20:48:17
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genhunter

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ºÎάÄÈ: ½ð±Ò+5, ¸ø5·Ö£¬Ôٸĸİɣ¡help help 2015-05-25 23:41:46
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In this study we prepared a reverse micelle system composed of TritonX-100/hexanol/cyclohexane/FITC in water (where was chitosan and TOES?). After 24hrs, acetone was added to break the emulsionm, fololowed by centrifuge, ultrasound treatment, FITC incorporated fluorescence SiO2 composite nanopartilces were prepared. We did optimization on the amounts of chitosan and dye that were used in the preparation,  and characterized the fluorescent properties of nanoparticles prepared. We found that there was an optimal amount of the dye content that was incorporated, and poorer results were obtained when it exceeded this amount.  Finally, we tested the quenching effect of chloride ion on the fluorescence from nanoparticles and demonstrated it can be used as a testing agent in a simple and straightforward manner.
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5Â¥2015-05-25 21:07:55
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genhunter

ÖÁ×ðľ³æ (ÖøÃûдÊÖ)

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slap, slap!

fololowed by centrifuge, ultrasound treatment-->   followed by centrifuge, ultrasound treatments
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6Â¥2015-05-25 21:20:15
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

genhunter

ÖÁ×ðľ³æ (ÖøÃûдÊÖ)

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In this study we prepared a reverse microemulsion system composed of TritonX-100/hexanol/cyclohexane/FITC in water (¿¼ÂÇÌí¼Óchitosan,TOES? ). After 24hrs, acetone was added to break the emulsion, FITC incorporated fluorescence SiO2 composite nanopartilces were obtained after being washed repeatedly using ultrasound treatment/centrifuge cycles. The contents of chitosan and dye were varied in the preparation to optimize the preparation,  and resulted nanoparticles were characterized on the fluorescence properties. We found that there was an optimal amount of the dye that was incorporated, and poorer results were obtained when it exceeded or lessened the optimum.  Finally, we tested the quenching effect of chloride ion on the fluorescence intensity of the nanoparticles. Our results demonstrated that  by using this simple and effective preparation protocol , fluorescence SiO2 composite nanopartilces can be readily prepared, which can be a useful testing agent for many analytic purposes.
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7Â¥2015-05-26 09:08:54
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

genhunter

ÖÁ×ðľ³æ (ÖøÃûдÊÖ)

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The contents of chitosan and dye were varied  to optimize the preparation
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8Â¥2015-05-26 09:24:08
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

genhunter

ÖÁ×ðľ³æ (ÖøÃûдÊÖ)

ÒýÓûØÌû:
8Â¥: Originally posted by genhunter at 2015-05-26 09:24:08
The contents of chitosan and dye were varied  to optimize the preparation

after being washed repeatedly using ultrasound treatment/centrifuge cycles-->

after repeated washes using ultrasound treatment/centrifuge cycles
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9Â¥2015-05-26 10:46:38
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