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13561217309

金虫 (小有名气)

[求助] 关于文献,有点看不懂。 已有1人参与

The large size of fibrinogen (340 kDa) and polymerized fibrin necessitates digestion prior to gel electrophoresis. Protein samples were digested with 9 M urea containing 9% β-mercaptoethanol and 3% SDS (50 μL) overnight at RT. Each sample (10 μL) was separated using a 3 −8% Tris-acetate gel
(Invitrogen) and proteins were stained with Coomassie Blue R-250.Gels were imaged using a Typhoon 9210 variable mode scanner (Amerisham Biosciences) and analyzed using ImageJ.其中的Protein samples were digested with 9 M urea containing 9% β-mercaptoethanol and 3% SDS (50 μL) overnight at RT. 是什么意思,溶液该怎么配制?
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  • 2014-10-07 21:40:06, 636.67 K

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biostar2009

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尿素和SDS都用来变性
beta-ME用来打开二硫键
为何叫“digestion”????
5楼2014-10-10 19:52:46
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焃__麟

铜虫 (初入文坛)

【答案】应助回帖

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感谢参与,应助指数 +1
13561217309(西门吹雪170代发): 金币+2, 鼓励回帖交流 2014-10-08 08:41:55
13561217309: 金币+3, ★★★★★最佳答案 2014-10-08 09:54:24
Protein samples were digested with 9 M urea containing 9% β-mercaptoethanol and 3% SDS (50 μL) overnight at RT
中文意思是:室温下用50μl的'9 M urea containing 9% β-mercaptoethanol and 3% SDS'溶液过夜处理该蛋白样品
至于该溶液怎么配制,人家不是告诉你成分和含量了么
行到水穷处,坐看云起时
2楼2014-10-07 22:33:53
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13561217309

金虫 (小有名气)

引用回帖:
2楼: Originally posted by 焃__麟 at 2014-10-07 22:33:53
Protein samples were digested with 9 M urea containing 9% β-mercaptoethanol and 3% SDS (50 μL) overnight at RT
中文意思是:室温下用50μl的'9 M urea containing 9% β-mercaptoethanol and 3% SDS'溶液 ...

就是不明白呢,

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3楼2014-10-08 09:19:02
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焃__麟

铜虫 (初入文坛)

这有什么不明白的?不是很清楚了么?
行到水穷处,坐看云起时
4楼2014-10-08 16:40:49
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