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wusiwl19

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[求助] 有高手可以翻译下这段吗? 已有1人参与

Considering the problem of plasma sample preparation, the solubilization of proteins instead of their precipitation prior SPE seems to be practically
more advantageous [5].
Inorder to revise this procedure, the effect of varying chaotropicity and concentration of salt-type additives has been recently examined [6].
The recovery results revealed that the addition of fairly diluted solutions containing chaotropic ions can be successfully applied for this purpose.
It should be emphasized that such enrichment of the plasma sample gave minimal matrix effect in further reverse phase (RP) HPLC analysis.
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大麦07

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【答案】应助回帖

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wusiwl19: 金币+5, ★★★★★最佳答案 2014-09-11 08:42:05
考虑到血浆样品的制样问题,在SPE前蛋白溶解比沉淀在实践上更具有优势[5]。
为了改进这一流程,最近对盐类添加剂的不同离液序列(chaotropicity)和浓度的效应进行了研究[6]。
回收率结果显示,加入相当稀的离液序列高的离子可以成功地用于实现这一目的。应当强调的是,这种血浆样品的富集方法在后面的反相HPLC分析中,基体效应最小。
2楼2014-09-10 19:47:27
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