24小时热门版块排行榜    

Znn3bq.jpeg
汕头大学海洋科学接受调剂
查看: 1826  |  回复: 7

baidiliulian

木虫 (小有名气)

[求助] S17大肠杆菌与假单胞菌的结合实验 已有3人参与

我用的是pk18mobsacB质粒载体转化到S17大肠杆菌中去的,S17和pk18mobsacB质粒都不带有壮观霉素的抗性,可是得到的带有pk18mobsacB质粒的S17大肠杆菌就有了壮观抗性,这是哪出错了?
回复此楼
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

zhaozhibozhi

金虫 (正式写手)

【答案】应助回帖

★ ★ ★ ★ ★
感谢参与,应助指数 +1
baidiliulian: 金币+5, ★★★★★最佳答案 2014-04-30 13:07:12
S17-1是有壮观霉素抗性的
做一个阳光、开朗的小和尚
2楼2014-04-27 22:26:20
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

baidiliulian

木虫 (小有名气)

引用回帖:
2楼: Originally posted by zhaozhibozhi at 2014-04-27 22:26:20
S17-1是有壮观霉素抗性的

S17-1确定是有壮观抗性的吗?我在网上没查到这方面的信息。谢谢!
3楼2014-04-28 10:07:16
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

zhaozhibozhi

金虫 (正式写手)

引用回帖:
3楼: Originally posted by baidiliulian at 2014-04-28 10:07:16
S17-1确定是有壮观抗性的吗?我在网上没查到这方面的信息。谢谢!...

ATCC说明:
ATCC® Number: 47055™ Price: $168.00
Designations: S17-1
Depositors: D Parke
Genotype: recA pro hsdR RP4-2-Tc::Mu-Km::Tn7
Growth Conditions: Protocol: plus trimethopirm (10 mcg/ml)
Temperature: 37.0°C
Applications: transformation host
Comments: Recommended mobilization host for pARO180, pARO190, pARO181, and pARO191 (ATCC 77123-77126, respectively) because it contains chromosomally integrated tra genes. [29601]
A kanamycin-sensitive strain devoid of the E. coli K-12-specific restriction system allowing efficient uptake of foreign DNA. [41566]
The properties of streptomycin, trimethoprim, and spectinomycin resistance were verified.
RP4-Tc::Mu-Km::Tn7 is integrated into the chromosome. [29601]
References: 29601: Parke D. Construction of mobilizable vectors derived from plasmids RP4, pUC18 and pUC19. Gene 93: 135-137, 1990. PubMed: 2227423
30953: Simon R, et al. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria. Bio-Technology 1: 784-791, 1983.
41566: McFarlane GJ, et al. A simplified method for conjugal gene transfer into the filamentous cyanobacterium Anabaena sp. ATCC 27893. J. Microbiol. Methods 6: 301-305, 1987.
做一个阳光、开朗的小和尚
4楼2014-04-29 09:51:39
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

baidiliulian

木虫 (小有名气)

引用回帖:
4楼: Originally posted by zhaozhibozhi at 2014-04-29 09:51:39
ATCC说明:
ATCC® Number: 47055™ Price: $168.00
Designations: S17-1
Depositors: D Parke
Genotype: recA pro hsdR RP4-2-Tc::Mu-Km::Tn7
Growth Conditions: Protocol: plus trimethop ...

十分感谢!我昨天也做了检测,发现确实有壮观抗性
5楼2014-04-29 22:44:33
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

兰炫

新虫 (初入文坛)

【答案】应助回帖

pk18mobsacB这个载体是含有卡那抗性的吧?怎么会有壮观霉素的抗性呢?
好好做实验!
6楼2014-07-12 15:03:14
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

baidiliulian

木虫 (小有名气)

引用回帖:
6楼: Originally posted by 兰炫 at 2014-07-12 15:03:14
pk18mobsacB这个载体是含有卡那抗性的吧?怎么会有壮观霉素的抗性呢?

s17菌有壮观抗性
7楼2014-07-13 13:28:34
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

北冰洋的雨

新虫 (初入文坛)

【答案】应助回帖

内容已删除
没有最好只有更好
8楼2015-10-16 11:29:54
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
相关版块跳转 我要订阅楼主 baidiliulian 的主题更新
最具人气热帖推荐 [查看全部] 作者 回/看 最后发表
[考研] 284求调剂 +16 让我上岸吧阿西 2026-04-09 16/800 2026-04-13 22:18 by pies112
[考研] 化学070300 求调剂 +21 哈哈哈^_^ 2026-04-12 21/1050 2026-04-13 21:36 by nxybio2007
[考研] 一志愿西交机械专硕求调剂 +9 求上岸的小王 2026-04-10 9/450 2026-04-13 16:08 by jiangguiquan11
[考研] 一志愿双非085400电子信息344 求调剂,对材料和化学方向也感兴趣 +12 无情的小羊 2026-04-09 13/650 2026-04-13 14:17 by 张zhihao
[考研] 材料299专硕求调剂 +13 +21 2026-04-09 13/650 2026-04-13 14:16 by 张zhihao
[考研] 考研二轮调剂 +10 故人?? 2026-04-09 10/500 2026-04-13 09:55 by szhize
[考研] 一志愿西北工业大学289 085602 +33 yang婷 2026-04-10 34/1700 2026-04-12 08:11 by Art1977
[考研] 296求调剂 +8 汪!?! 2026-04-09 8/400 2026-04-11 21:02 by 逆水乘风
[考研] 调剂 化学 307 +21 73372112 2026-04-09 23/1150 2026-04-10 23:53 by wj165256
[考研] 考研调剂 +26 硕星赴 2026-04-09 27/1350 2026-04-10 22:24 by 猪会飞
[考研] 085404 298分求调剂 +10 呼啦呼啦呼呼呼 2026-04-10 11/550 2026-04-10 16:44 by wangy0907
[论文投稿] mdpi小修rvr时间四五天了 20+3 哈哈high 2026-04-08 5/250 2026-04-10 16:02 by 北京莱茵润色
[考研] 江苏大学 工科调剂 捡漏 +3 Evan_Liu 2026-04-09 5/250 2026-04-10 10:22 by Evan_Liu
[考研] 一志愿中科院105500专业总分315求调剂 +6 lallalh 2026-04-09 7/350 2026-04-09 17:51 by lallalh
[考研] 求调剂,现在还能填的 +3 上岸小莹加油 2026-04-08 3/150 2026-04-08 14:30 by zhq0425
[考研] 263分B区求调剂 +6 李nihao 2026-04-08 6/300 2026-04-08 09:38 by 南开小綦
[考研] 313求调剂 +3 十六拾陆 2026-04-07 3/150 2026-04-07 23:20 by lbsjt
[考研] 计算机11408 287 求调剂 +3 LiLe5 2026-04-07 3/150 2026-04-07 23:15 by shanqishi
[考研] 316求调剂 +4 15318418673 2026-04-07 4/200 2026-04-07 22:12 by hemengdong
[考研] 专硕085403,291分,有两篇专利,一国一奖 +3 哈吉咪哈吉咪 2026-04-07 3/150 2026-04-07 18:21 by 蓝云思雨
信息提示
请填处理意见