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baidiliulian

木虫 (小有名气)

[求助] S17大肠杆菌与假单胞菌的结合实验 已有3人参与

我用的是pk18mobsacB质粒载体转化到S17大肠杆菌中去的,S17和pk18mobsacB质粒都不带有壮观霉素的抗性,可是得到的带有pk18mobsacB质粒的S17大肠杆菌就有了壮观抗性,这是哪出错了?
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兰炫

新虫 (初入文坛)

【答案】应助回帖

pk18mobsacB这个载体是含有卡那抗性的吧?怎么会有壮观霉素的抗性呢?
好好做实验!
6楼2014-07-12 15:03:14
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zhaozhibozhi

金虫 (正式写手)

【答案】应助回帖

★ ★ ★ ★ ★
感谢参与,应助指数 +1
baidiliulian: 金币+5, ★★★★★最佳答案 2014-04-30 13:07:12
S17-1是有壮观霉素抗性的
做一个阳光、开朗的小和尚
2楼2014-04-27 22:26:20
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baidiliulian

木虫 (小有名气)

引用回帖:
2楼: Originally posted by zhaozhibozhi at 2014-04-27 22:26:20
S17-1是有壮观霉素抗性的

S17-1确定是有壮观抗性的吗?我在网上没查到这方面的信息。谢谢!
3楼2014-04-28 10:07:16
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zhaozhibozhi

金虫 (正式写手)

引用回帖:
3楼: Originally posted by baidiliulian at 2014-04-28 10:07:16
S17-1确定是有壮观抗性的吗?我在网上没查到这方面的信息。谢谢!...

ATCC说明:
ATCC® Number: 47055™ Price: $168.00
Designations: S17-1
Depositors: D Parke
Genotype: recA pro hsdR RP4-2-Tc::Mu-Km::Tn7
Growth Conditions: Protocol: plus trimethopirm (10 mcg/ml)
Temperature: 37.0°C
Applications: transformation host
Comments: Recommended mobilization host for pARO180, pARO190, pARO181, and pARO191 (ATCC 77123-77126, respectively) because it contains chromosomally integrated tra genes. [29601]
A kanamycin-sensitive strain devoid of the E. coli K-12-specific restriction system allowing efficient uptake of foreign DNA. [41566]
The properties of streptomycin, trimethoprim, and spectinomycin resistance were verified.
RP4-Tc::Mu-Km::Tn7 is integrated into the chromosome. [29601]
References: 29601: Parke D. Construction of mobilizable vectors derived from plasmids RP4, pUC18 and pUC19. Gene 93: 135-137, 1990. PubMed: 2227423
30953: Simon R, et al. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria. Bio-Technology 1: 784-791, 1983.
41566: McFarlane GJ, et al. A simplified method for conjugal gene transfer into the filamentous cyanobacterium Anabaena sp. ATCC 27893. J. Microbiol. Methods 6: 301-305, 1987.
做一个阳光、开朗的小和尚
4楼2014-04-29 09:51:39
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