24小时热门版块排行榜    

查看: 1772  |  回复: 7
当前只显示满足指定条件的回帖,点击这里查看本话题的所有回帖

baidiliulian

木虫 (小有名气)

[求助] S17大肠杆菌与假单胞菌的结合实验 已有3人参与

我用的是pk18mobsacB质粒载体转化到S17大肠杆菌中去的,S17和pk18mobsacB质粒都不带有壮观霉素的抗性,可是得到的带有pk18mobsacB质粒的S17大肠杆菌就有了壮观抗性,这是哪出错了?
回复此楼
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

zhaozhibozhi

金虫 (正式写手)

引用回帖:
3楼: Originally posted by baidiliulian at 2014-04-28 10:07:16
S17-1确定是有壮观抗性的吗?我在网上没查到这方面的信息。谢谢!...

ATCC说明:
ATCC® Number: 47055™ Price: $168.00
Designations: S17-1
Depositors: D Parke
Genotype: recA pro hsdR RP4-2-Tc::Mu-Km::Tn7
Growth Conditions: Protocol: plus trimethopirm (10 mcg/ml)
Temperature: 37.0°C
Applications: transformation host
Comments: Recommended mobilization host for pARO180, pARO190, pARO181, and pARO191 (ATCC 77123-77126, respectively) because it contains chromosomally integrated tra genes. [29601]
A kanamycin-sensitive strain devoid of the E. coli K-12-specific restriction system allowing efficient uptake of foreign DNA. [41566]
The properties of streptomycin, trimethoprim, and spectinomycin resistance were verified.
RP4-Tc::Mu-Km::Tn7 is integrated into the chromosome. [29601]
References: 29601: Parke D. Construction of mobilizable vectors derived from plasmids RP4, pUC18 and pUC19. Gene 93: 135-137, 1990. PubMed: 2227423
30953: Simon R, et al. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria. Bio-Technology 1: 784-791, 1983.
41566: McFarlane GJ, et al. A simplified method for conjugal gene transfer into the filamentous cyanobacterium Anabaena sp. ATCC 27893. J. Microbiol. Methods 6: 301-305, 1987.
做一个阳光、开朗的小和尚
4楼2014-04-29 09:51:39
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
查看全部 8 个回答

zhaozhibozhi

金虫 (正式写手)

【答案】应助回帖

★ ★ ★ ★ ★
感谢参与,应助指数 +1
baidiliulian: 金币+5, ★★★★★最佳答案 2014-04-30 13:07:12
S17-1是有壮观霉素抗性的
做一个阳光、开朗的小和尚
2楼2014-04-27 22:26:20
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

baidiliulian

木虫 (小有名气)

引用回帖:
2楼: Originally posted by zhaozhibozhi at 2014-04-27 22:26:20
S17-1是有壮观霉素抗性的

S17-1确定是有壮观抗性的吗?我在网上没查到这方面的信息。谢谢!
3楼2014-04-28 10:07:16
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

baidiliulian

木虫 (小有名气)

引用回帖:
4楼: Originally posted by zhaozhibozhi at 2014-04-29 09:51:39
ATCC说明:
ATCC® Number: 47055™ Price: $168.00
Designations: S17-1
Depositors: D Parke
Genotype: recA pro hsdR RP4-2-Tc::Mu-Km::Tn7
Growth Conditions: Protocol: plus trimethop ...

十分感谢!我昨天也做了检测,发现确实有壮观抗性
5楼2014-04-29 22:44:33
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
最具人气热帖推荐 [查看全部] 作者 回/看 最后发表
[考研] 0856材料求调剂 +12 hyf hyf hyf 2026-02-28 13/650 2026-03-02 20:19 by hypershenger
[考研] 085600材料工程一志愿中科大总分312求调剂 +9 吃宵夜1 2026-02-28 11/550 2026-03-02 20:14 by hypershenger
[考研] 271求调剂 +3 Ricardo1113 2026-03-02 3/150 2026-03-02 19:53 by zhukairuo
[考研] 0805总分292,求调剂 +10 幻想之殇 2026-03-01 10/500 2026-03-02 19:51 by zhukairuo
[考研] 268求调剂 +3 好运连绵不绝 2026-03-02 3/150 2026-03-02 19:05 by zhukairuo
[考研] 一志愿华中科技大学,化学专业344分,求调剂 +3 邢xing1 2026-03-02 3/150 2026-03-02 17:32 by houyaoxu
[考研] 一志愿华南理工大学材料与化工326分,求调剂 +3 wujinrui1 2026-02-28 3/150 2026-03-02 16:36 by chuocheng
[考研] 338求调剂 +3 18162027187 2026-03-02 3/150 2026-03-02 13:12 by houyaoxu
[考研] 材料与化工328求调剂 +3 。,。,。,。i 2026-03-02 3/150 2026-03-02 13:09 by houyaoxu
[考研] 26考研报考西工大材料308分求调剂 +4 weizhong123 2026-03-01 4/200 2026-03-02 12:46 by 无际的草原
[考研] 求调剂 +3 熬夜的猫头鹰 2026-03-02 3/150 2026-03-02 11:45 by 刘兵
[考研] 0856材料调剂 +4 沿岸有贝壳OUC 2026-03-02 4/200 2026-03-02 10:19 by 公瑾逍遥
[考研] 322求调剂 +3 熊境喆 2026-03-01 3/150 2026-03-02 08:44 by houyaoxu
[考研] 0857调剂 +4 一ll半 2026-02-28 5/250 2026-03-02 02:33 by 908055542
[基金申请] 成果系统访问量大,请一小时后再尝试。---NSFC啥时候好哦,已经两天这样了 +4 NSFC2026我来了 2026-02-28 4/200 2026-03-01 22:37 by 铁门栓
[考研] 272求调剂 +6 田智友 2026-02-28 6/300 2026-03-01 21:40 by 公瑾逍遥
[考研] 化工299分求调剂 一志愿985落榜 +5 嘻嘻(*^ω^*) 2026-03-01 5/250 2026-03-01 19:47 by 无际的草原
[考研] 285求调剂 +8 满头大汗的学生 2026-02-28 8/400 2026-03-01 16:47 by caszguilin
[考研] 求调剂 +6 repeatt?t 2026-02-28 6/300 2026-03-01 14:37 by Sakura绘
[考研] 295复试调剂 +3 简木ChuFront 2026-03-01 3/150 2026-03-01 14:27 by zzxw520th
信息提示
请填处理意见