24小时热门版块排行榜    

查看: 1773  |  回复: 7
当前只显示满足指定条件的回帖,点击这里查看本话题的所有回帖

baidiliulian

木虫 (小有名气)

[求助] S17大肠杆菌与假单胞菌的结合实验 已有3人参与

我用的是pk18mobsacB质粒载体转化到S17大肠杆菌中去的,S17和pk18mobsacB质粒都不带有壮观霉素的抗性,可是得到的带有pk18mobsacB质粒的S17大肠杆菌就有了壮观抗性,这是哪出错了?
回复此楼
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

baidiliulian

木虫 (小有名气)

引用回帖:
6楼: Originally posted by 兰炫 at 2014-07-12 15:03:14
pk18mobsacB这个载体是含有卡那抗性的吧?怎么会有壮观霉素的抗性呢?

s17菌有壮观抗性
7楼2014-07-13 13:28:34
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
查看全部 8 个回答

zhaozhibozhi

金虫 (正式写手)

【答案】应助回帖

★ ★ ★ ★ ★
感谢参与,应助指数 +1
baidiliulian: 金币+5, ★★★★★最佳答案 2014-04-30 13:07:12
S17-1是有壮观霉素抗性的
做一个阳光、开朗的小和尚
2楼2014-04-27 22:26:20
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

baidiliulian

木虫 (小有名气)

引用回帖:
2楼: Originally posted by zhaozhibozhi at 2014-04-27 22:26:20
S17-1是有壮观霉素抗性的

S17-1确定是有壮观抗性的吗?我在网上没查到这方面的信息。谢谢!
3楼2014-04-28 10:07:16
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

zhaozhibozhi

金虫 (正式写手)

引用回帖:
3楼: Originally posted by baidiliulian at 2014-04-28 10:07:16
S17-1确定是有壮观抗性的吗?我在网上没查到这方面的信息。谢谢!...

ATCC说明:
ATCC® Number: 47055™ Price: $168.00
Designations: S17-1
Depositors: D Parke
Genotype: recA pro hsdR RP4-2-Tc::Mu-Km::Tn7
Growth Conditions: Protocol: plus trimethopirm (10 mcg/ml)
Temperature: 37.0°C
Applications: transformation host
Comments: Recommended mobilization host for pARO180, pARO190, pARO181, and pARO191 (ATCC 77123-77126, respectively) because it contains chromosomally integrated tra genes. [29601]
A kanamycin-sensitive strain devoid of the E. coli K-12-specific restriction system allowing efficient uptake of foreign DNA. [41566]
The properties of streptomycin, trimethoprim, and spectinomycin resistance were verified.
RP4-Tc::Mu-Km::Tn7 is integrated into the chromosome. [29601]
References: 29601: Parke D. Construction of mobilizable vectors derived from plasmids RP4, pUC18 and pUC19. Gene 93: 135-137, 1990. PubMed: 2227423
30953: Simon R, et al. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria. Bio-Technology 1: 784-791, 1983.
41566: McFarlane GJ, et al. A simplified method for conjugal gene transfer into the filamentous cyanobacterium Anabaena sp. ATCC 27893. J. Microbiol. Methods 6: 301-305, 1987.
做一个阳光、开朗的小和尚
4楼2014-04-29 09:51:39
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
最具人气热帖推荐 [查看全部] 作者 回/看 最后发表
[考研] 0856材料求调剂 +12 hyf hyf hyf 2026-02-28 13/650 2026-03-02 20:19 by hypershenger
[考研] 材料284求调剂,一志愿郑州大学英一数二专硕 +15 想上岸的土拨鼠 2026-02-28 15/750 2026-03-02 20:13 by hypershenger
[考研] 材料复试调剂 +5 学材料的点 2026-03-01 6/300 2026-03-02 20:01 by hypershenger
[考研] 调剂 +3 13853210211 2026-03-02 3/150 2026-03-02 19:59 by hypershenger
[考研] 0854总分272 +3 打小就是老实人 2026-03-02 4/200 2026-03-02 19:49 by 求调剂zz
[考研] 283求调剂 +5 鹿沫笙 2026-03-02 5/250 2026-03-02 19:19 by zhukairuo
[考研] 085600 英一数二272求调剂 5+6 vida_a 2026-03-01 16/800 2026-03-02 19:13 by zhukairuo
[考研] 一志愿华中科技大学,化学专业344分,求调剂 +3 邢xing1 2026-03-02 3/150 2026-03-02 17:32 by houyaoxu
[考研] 289求调剂 +3 BrightLL 2026-03-02 3/150 2026-03-02 17:28 by 杨杨杨紫
[考研] 303求调剂 +5 今夏不夏 2026-03-01 5/250 2026-03-02 15:01 by 向上的胖东
[考研] 292求调剂 +7 yhk_819 2026-02-28 7/350 2026-03-02 12:43 by 无际的草原
[基金申请] 成果系统访问量大,请15分钟后再尝试。由此给您造成的不便,敬请谅解。 +5 xhuama 2026-03-02 5/250 2026-03-02 12:34 by stidwellNK
[考研] 295求调剂 +8 19171856320 2026-02-28 8/400 2026-03-02 11:19 by yuchj
[考研] 一志愿郑大材料学硕298分,求调剂 +6 wsl111 2026-03-01 6/300 2026-03-02 11:00 by ydudjddnd
[考研] 264求调剂 +4 巴拉巴拉根556 2026-02-28 4/200 2026-03-02 10:48 by yuchj
[考研] 275求调剂 +3 L-xin? 2026-03-01 6/300 2026-03-02 10:22 by 热情沙漠
[考研] 0856材料调剂 +4 沿岸有贝壳OUC 2026-03-02 4/200 2026-03-02 10:19 by 公瑾逍遥
[基金申请] 成果系统访问量大,请一小时后再尝试。---NSFC啥时候好哦,已经两天这样了 +4 NSFC2026我来了 2026-02-28 4/200 2026-03-01 22:37 by 铁门栓
[考研] 299求调剂 +3 Y墨明棋妙Y 2026-02-28 5/250 2026-03-01 21:01 by tangxiaotian
[论文投稿] Optics letters投稿被拒求助 30+3 luckyry 2026-02-26 4/200 2026-03-01 09:06 by babero
信息提示
请填处理意见