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铜虫 (小有名气)

[求助] 【求各位科学家帮助】sci翻译 英译汉

2.5. Analysis of the composition of SFP
The HPLC method was used to identify the monosaccharides
in SFP [11]. The sample (10 mg) of SFP was hydrolyzed with
2 mol/l H2SO4 (2 ml) at 100 ◦C for 5 h, and the excess acid was
neutralized with barium carbonate. After standing overnight, the
neutral products were separated by centrifugation at 3000 rpm
for 10 min, 100 l supernatant was collected and reacted with 1-
phenyl-3-methyl-5-pyrazolone, then subsequently separated by
HPLC (Shimadzu LC-10AT VP, Japan) using a Packed column
(4.6 × 250 mm) with detection at 250 nm. The mobile phase were
consisted of 78% phosphate buffer solution (0.05 M, KH2PO4-NaOH,
pH 6.9) and 22% acetonitrile (v/v), and the samples were eluted at a
flow rate of 1.0 ml/min at 28 ◦C. In this study, l-Rha, d-Gal, d-GalA,
d-Glu and Ara were used as references.

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youxi2

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2.5.SFP 的组成的分析高效液相色谱法用来标识单糖组成的在 SFP [11]。示例 (10 毫克) 的 SFP 被水解与2 mol/l h2 如此 4 (2 毫升) 100 ◦C 5 h 和过剩的酸是与碳酸钡中立化。后站在一夜之间,中性产品被用在 3000 rpm 离心法分离10 分钟,100 升上清液收集并与 1-的反应苯基-3-甲基-5-吡唑酮,然后随后隔开高效液相色谱法日本岛津 LC-10AT 副总裁) 使用填充的柱(4.6 × 250 毫米) 与检测在 250 毫微米。流动相了包括了 78%磷酸缓冲溶液 (0.05 M,KH2PO4-NaOH,pH 6.9) 和乙腈 22%(v/v) 和样品被洗脱在28 ◦C 1.0 ml/min 的流量速率。在此研究,l Rha,d-半乳糖,d-联欢晚会d-谷氨酸和 Ara 被用作参考。
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