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2.5. Analysis of the composition of SFP The HPLC method was used to identify the monosaccharides in SFP [11]. The sample (10 mg) of SFP was hydrolyzed with 2 mol/l H2SO4 (2 ml) at 100 ◦C for 5 h, and the excess acid was neutralized with barium carbonate. After standing overnight, the neutral products were separated by centrifugation at 3000 rpm for 10 min, 100 l supernatant was collected and reacted with 1- phenyl-3-methyl-5-pyrazolone, then subsequently separated by HPLC (Shimadzu LC-10AT VP, Japan) using a Packed column (4.6 ¡Á 250 mm) with detection at 250 nm. The mobile phase were consisted of 78% phosphate buffer solution (0.05 M, KH2PO4-NaOH, pH 6.9) and 22% acetonitrile (v/v), and the samples were eluted at a flow rate of 1.0 ml/min at 28 ◦C. In this study, l-Rha, d-Gal, d-GalA, d-Glu and Ara were used as references. |
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