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rafy01

金虫 (正式写手)

[求助] 求教:牛奶中蛋白质的去除

http://www.sciencedirect.com/sci ... i/S0039914010005655
这篇文献实验部分中提到,牛奶中杂质(应该是蛋白质氨基酸)的去除过程:先加入三氯乙酸和氯仿,离心去除沉淀;再用碳酸钠调节ph,再次离心去除沉淀。文献中并未提及每步所去除的具体杂质?为何第二步还要调节ph之后再进行去除?是否在不同ph下所去除的氨基酸种类不同呢?
非生物专业,最好有引用的参考文献。。。
原文:For the detection of liquid milk samples, about 1.6 g sample was
weighed for the determination. The sample was firstly mixed with
2.5 mL of water, 0.7 mL of 10% trichloroacetic acid and 1.0 mL of
chloroform. Then, the mixture was ultrasonically treated for 15 min
and centrifuged at 13,000 rmp for 6 min to separate the deposit. The
solution was transferred into another centrifuge tube and adjusted
to pH 8.0 with 1 M of Na2CO3 solution. The solution was centrifuged at 3000 rmp for 1 min to remove the deposit again and the final
solution was used for detection.
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小木虫
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buggiegoat

新虫 (初入文坛)

【答案】应助回帖

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牛奶蛋白里有whey protein等蛋白,每步将每种蛋白去除。

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2楼2013-02-01 20:01:15
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rafy01

金虫 (正式写手)

引用回帖:
2楼: Originally posted by buggiegoat at 2013-02-01 20:01:15
牛奶蛋白里有whey protein等蛋白,每步将每种蛋白去除。

能否具体描述?有文献依据吗?
小木虫
3楼2013-02-02 10:34:26
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ghqiu09

禁虫 (正式写手)

★ ★
感谢参与,应助指数 +1
wizardfan: 金币+2, 谢谢参与 2013-02-03 09:26:10
本帖内容被屏蔽

4楼2013-02-03 08:01:36
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siyianhong

银虫 (小有名气)

【答案】应助回帖

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感谢参与,应助指数 +1
wizardfan: 金币+2, 热变性是种办法 2013-02-03 09:25:47
煮一煮,离心过滤,去得干干净净。

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5楼2013-02-03 08:39:24
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wizardfan

至尊木虫 (著名写手)

优秀版主

【答案】应助回帖

感谢参与,应助指数 +1
直接问作者就可以了。
还有看到你没有提到超声波一步,提醒下
6楼2013-02-03 09:25:18
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